Protection against neonatal enteric colibacillosis employing E. Coli-derived outer membrane vesicles in formulation and without vitamin D3

BMC Research Notes
Babak Beikzadeh, Gholamreza Nikbakht Brujeni

Abstract

Enterotoxigenic Escherichia Coli (ETEC) is the cause of diarrhea and even death in humans and offspring of animals. Outer membrane vesicles (OMVs) of the ETEC was prepared and its potential as a vaccine candidate against enteric colibacillosis in neonatal mice was evaluated. Dam mice intradermally injected with ETEC-derived OMVs and OMVs plus an active form of vitamin D3 (avD3). Mucosal and systemic immune responses in mice and passive immunity protection against ETEC lethality in their offspring was investigated. Immunization of adult mice via ETEC-derived OMV alone and in formulation with avD3 protect offspring from ETEC-induced lethality. Nevertheless, avD3 did not indicate a positive effect on mucosal and systemic immune responses. Only the combination of OMV plus avD3 elicited a significant (P < 0.05) increase in the level of specific IgA antibodies in serum.

References

Jan 1, 1986·Journal of Clinical Immunology·D J SmithJ L Ebersole
Jul 1, 1971·The New England Journal of Medicine·H L DuPontJ P Kalas
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Mar 30, 2002·Trends in Immunology·Jean Pierre BouvetPerayot Pamonsinlapatham
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·Béla Nagy, Péter Z Fekete
Jan 21, 2006·The Journal of Infectious Diseases·Alexander P IvanovKonstantin M Chumakov
Aug 6, 2008·Infection and Immunity·Stefan SchildAndrew Camilli
May 12, 2010·Infection and Immunity·Koushik RoyJames M Fleckenstein
Dec 15, 2010·Seminars in Immunopathology·Can M UnalKristian Riesbeck
Jan 22, 2011·Current Topics in Microbiology and Immunology·N RomaniP Stoitzner
Mar 22, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oh Youn KimYong Song Gho
Jun 27, 2013·Frontiers in Immunology·Cynthia M FehresYvette van Kooyk
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Jun 11, 2014·International Immunology·Tatsuhiko AzegamiHiroshi Kiyono
Jul 30, 2014·Critical Reviews in Therapeutic Drug Carrier Systems·Hinal PatelAmbikanandan Misra
Jan 30, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Atul SrivastavaMeenakshi Iyer
Mar 19, 2015·Frontiers in Immunology·Gabriela Andrea Pizzurro, María Marcela Barrio
Sep 1, 2015·Frontiers in Microbiology·Deborah R LeitnerStefan Schild
Feb 9, 2017·Clinical and Experimental Vaccine Research·Sae-Hae Kim, Yong-Suk Jang

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
lavage
enzyme-link immunosorbent assay
PCR
electrophoresis
Transmission

Software Mentioned

ZEN lite

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.