PMID: 8611642Jan 17, 1996Paper

Protection against oxidative injury and permeability alteration in cultured alveolar epithelium by transferrin-catalase conjugate

Biochimica Et Biophysica Acta
Y RojanasakulL Y Wang

Abstract

The successful prevention of hydrogen peroxide-induced alveolar permeability alterations and cell injury by transferrin-catalase conjugate is described in this study. Permeability alterations and cell injury were induced in cultured alveolar epithelial monolayers by hydrogen peroxide. Transepithelial transport of a permeability marker, [14C] mannitol, and cellular nuclear fluorescence of a membrane integrity indicator, propidium iodide, were used to quantitate epithelial permeability and damage respectively. Hydrogen peroxide (0.1 - 10 mM) induced a dose-dependent increase in both alveolar permeability and cellular damage; however, the oxidant effect on monolayer permeability did not require prior cell damage. Electron spin resonance measurements using the spin trap 5,5-dimethyl-l-pyrroline-N-oxide indicated the formation of hydroxyl radicals in hydrogen peroxide-treated cells. Chelation of the cellular pool of iron by deferoxamine inhibited radical formation and helped protect the cells from oxidative changes. Prior treatment of the cells with catalase (0.1 U-10 U/ml) had minimal protective effects on cell injury and permeability alterations. In contrast, transferrin-catalase conjugate, at the same concentration range, exhibit...Continue Reading

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Citations

Jan 5, 2002·Comprehensive Therapy·D P Pandya
Apr 17, 2002·International Journal of Pharmaceutics·Franz GaborMichael Wirth
Apr 9, 2001·Antioxidants & Redox Signaling·V R Muzykantov
Jul 6, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takayuki NemotoMitsuru Hashida
Jan 18, 2006·Treatments in Respiratory Medicine·Melpo Christofidou-Solomidou, Vladimir R Muzykantov
Jul 14, 2000·Physiological Reviews·B K Pedersen, L Hoffman-Goetz

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