Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus)

Fish & Shellfish Immunology
P PereiroA Estepa

Abstract

Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV(860)) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G(860)). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV(860) lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization.

References

Apr 24, 2004·The Journal of General Virology·Katja Einer-JensenNiels Lorenzen
Mar 11, 2005·Expert Review of Vaccines·Ingunn SommersetPetter Frost
Apr 26, 2006·Applied Microbiology and Biotechnology·I BrocalA Estepa
Jan 15, 2011·Fish & Shellfish Immunology·Marta AlonsoJo-Ann Leong

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Citations

Jun 16, 2016·Frontiers in Physiology·Patricia PereiroBeatriz Novoa
Nov 23, 2017·Frontiers in Immunology·Patricia PereiroBeatriz Novoa

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