Protection and recycling of alpha-tocopherol in human erythrocytes by intracellular ascorbic acid

Archives of Biochemistry and Biophysics
J M MayS Mendiratta

Abstract

Ascorbic acid can recycle alpha-tocopherol from the tocopheroxyl free radical in lipid bilayers and in micelles, but such recycling has not been demonstrated to occur across cell membranes. In this work the ability of intracellular ascorbate to protect and to recycle alpha-tocopherol in intact human erythrocytes and erythrocyte ghosts was investigated. In erythrocytes that were 80% depleted of intracellular ascorbate by treatment with the nitroxide Tempol, both 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and ferricyanide oxidized alpha-tocopherol to a greater extent than in cells not depleted of ascorbate. In contrast, in erythrocytes in which the intracellular ascorbate concentration had been increased by loading with dehydroascorbate, loss of alpha-tocopherol was less with both oxidants than in control cells. Protection against AAPH-induced oxidation of alpha-tocopherol was not prevented by extracellular ascorbate oxidase, indicating that the protection was due to intracellular and not to extracellular ascorbate. Incubation of erythrocytes with lecithin liposomes also generated an oxidant stress, which caused lipid peroxidation in the liposomes and depleted erythrocyte alpha-tocopherol, leading to hemolysis. Ascorbat...Continue Reading

References

Jan 1, 1979·The Journal of Clinical Investigation·E P Orringer, M E Roer
Jan 1, 1979·Methods in Enzymology·L A Pachla, P T Kissinger
Jan 1, 1978·Journal of Nutritional Science and Vitaminology·M MinoY Yuguchi
May 1, 1977·The American Journal of Clinical Nutrition·J G BieriS Thorp
Aug 1, 1975·Archives of Biochemistry and Biophysics·H Shimasaki, O S Privett
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·I L SunH Löw
Jul 1, 1987·Chemistry and Physics of Lipids·E Niki
Nov 1, 1987·Chemistry and Physics of Lipids·M R Clemens, H D Waller
Aug 7, 1987·Biochimica Et Biophysica Acta·E S WagnerK Bennett
Mar 1, 1968·British Journal of Haematology·R E Hughes, S C Maton
Jan 1, 1968·Vitamins and Hormones·M K HorwittE M Harmon
Nov 1, 1969·The Journal of Clinical Investigation·R SilberH J Kayden
Nov 10, 1995·Archives of Biochemistry and Biophysics·D A StoyanovskyV E Kagan
Dec 1, 1995·The American Journal of Clinical Nutrition·S R ThomasR Stocker
Feb 20, 1995·Archives of Biochemistry and Biophysics·R H Bisby, A W Parker
Aug 4, 1993·Biochimica Et Biophysica Acta·E N IheanachoN H Hunt
Jan 1, 1996·Free Radical Biology & Medicine·J M MayC E Cobb
Dec 1, 1963·Analytical Biochemistry·M AVRON, N SHAVIT
Dec 1, 1969·The Journal of Membrane Biology·R K Mishra, H Passow

❮ Previous
Next ❯

Citations

Jan 17, 2009·Molecular and Cellular Biochemistry·James M May, Zhi-chao Qu
Sep 1, 2000·The British Journal of Nutrition·I M HamiltonJ J Strain
Dec 22, 2011·Nature Communications·Amber C HowardPaul L McNeil
Nov 30, 2005·The Proceedings of the Nutrition Society·Valerie A Holmes, David R McCance
Jan 30, 2002·Antioxidants & Redox Signaling·X LiJ M May
Mar 7, 2001·Antioxidants & Redox Signaling·J M May, Z C Qu
Aug 17, 2006·Antioxidants & Redox Signaling·Argirios E TsantesNikolaos M Sitaras
Sep 4, 2010·Antioxidants & Redox Signaling·Domenico Del PrincipeMaria Valeria Catani
Apr 16, 2013·Antioxidants & Redox Signaling·James M May, Fiona E Harrison
Jun 21, 2005·Plant Physiology·Hiroshi MaedaDean Dellapenna
Dec 30, 2008·Pharmacological Reviews·Christopher S Wilcox, Adam Pearlman
Mar 17, 2001·Journal of Clinical Pathology·I S Young, J V Woodside
May 8, 2009·American Journal of Physiology. Cell Physiology·James M MayHuan Qiao
Jun 30, 2011·Cell & Bioscience·Joachim PereraNagaraja Haleagrahara
Nov 8, 2005·Chemical & Pharmaceutical Bulletin·Cuiping ChiKazunori Anzai
Jul 5, 2008·Journal of Perinatal Medicine·Olivera Kontic-VucinicNebojsa Radunovic
Jul 31, 2013·Nutrients·Maiken LindbladJens Lykkesfeldt
Jul 31, 2014·Blood Transfusion = Trasfusione Del Sangue·Valeria PallottaLello Zolla
Aug 13, 2013·BioMed Research International·Ramón RodrigoDaniel Hasson
Aug 29, 2014·Canadian Journal of Physiology and Pharmacology·Rania H Abdou, Mohamed M Abdel-Daim
May 11, 2013·Clinical and Experimental Hypertension : CHE·Ramón RodrigoLeonardo Vergara
Nov 22, 2015·Molecular and Cellular Biochemistry·Esad UlkerJames M May
Dec 4, 2015·International Journal of Molecular Sciences·Adriana Covarrubias-PintoMaite Aintzane Castro
Jul 31, 2012·The Journal of Nutritional Biochemistry·Laura A Da CostaAhmed El-Sohemy
Apr 24, 2012·Analytical Biochemistry·Hongyan LiMark Levine
Dec 16, 2010·Biochemical and Biophysical Research Communications·James M May, Zhi-Chao Qu
Jan 24, 2009·Free Radical Biology & Medicine·Fiona E Harrison, James M May
Jul 5, 2008·Research in Veterinary Science·Mostafa A Saleh
Jun 28, 2008·Pharmacology & Therapeutics·Rene Aguirre, James M May
Mar 22, 2005·European Journal of Haematology·Fausta Omodeo-SalèDonatella Taramelli
Jun 1, 2005·BJOG : an International Journal of Obstetrics and Gynaecology·William D FraserPierre Julien

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.