Protection by meningococcal outer membrane protein PorA-specific antibodies and a serogroup B capsular polysaccharide-specific antibody in complement-sufficient and C6-deficient infant rats.

Infection and Immunity
M ToropainenHelena Käyhty

Abstract

The relative contributions of antibody-induced complement-mediated bacterial lysis and antibody/complement-mediated phagocytosis to host immunity against meningococcal infections are currently unclear. Further, the in vivo effector functions of antibodies may vary depending on their specificity and Fc heavy-chain isotype. In this study, a mouse immunoglobulin G2a (mIgG2a) monoclonal antibody (MN12H2) to meningococcal outer membrane protein PorA (P1.16), its human IgG subclass derivatives (hIgG1 to hIgG4), and an mIgG2a monoclonal antibody (Nmb735) to serogroup B capsular polysaccharide (B-PS) were evaluated for passive protection against meningococcal serogroup B strain 44/76-SL (B:15:P1.7,16) in an infant rat infection model. Complement component C6-deficient (PVG/c-) rats were used to assess the importance of complement-mediated bacterial lysis for protection. The PorA-specific parental mIgG2a and the hIgG1 to hIgG3 derivatives all induced efficient bactericidal activity in vitro in the presence of human or infant rat complement and augmented bacterial clearance in complement-sufficient HsdBrlHan:WIST rats, while the hIgG4 was unable to do so. In C6-deficient PVG/c- rats, lacking complement-mediated bacterial lysis, the augme...Continue Reading

References

Aug 1, 1977·The Journal of Infectious Diseases·R GoldE C Gotschlich
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·A HalstensenC O Solberg
Dec 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·H SjursenC O Solberg
Mar 1, 1988·Microbial Pathogenesis·K Saukkonen
Jun 1, 1969·The Journal of Experimental Medicine·I GoldschneiderM S Artenstein
Dec 1, 1984·Infection and Immunity·A HalstensenC O Solberg
Aug 1, 1994·The Journal of Infectious Diseases·M SchlesingerR Levy
Sep 1, 1994·Clinical and Experimental Immunology·P L LeenaertsM R Daha
Jan 1, 1993·Immunologic Research·J FigueroaP Densen
Nov 3, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A E PlatonovE J Kuijper
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G VidarssonJ G van De Winkel
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jo Anne WelschDan M Granoff
Jun 18, 2004·Emerging Infectious Diseases·Joanne E RussellJanet Suker

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Citations

Jan 19, 2010·World Journal of Gastroenterology : WJG·Maritza LienlafAlejandro Venegas
Jul 20, 2007·Clinical and Vaccine Immunology : CVI·Synne SandbuEinar Rosenqvist
Nov 13, 2020·Scientific Reports·Diane SuAileen J Anderson

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