PMID: 7541291Aug 1, 1995Paper

Protection of a T-cell line from human immunodeficiency virus replication by the stable expression of a short antisense RNA sequence carried by a shuttle RNA molecule

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
L CagnonA Doglio

Abstract

Adenovirus VA1 gene is efficiently transcribed by RNA polymerase III and gives rise to a small highly ordered RNA. To inhibit replication of human immunodeficiency virus (HIV), a chimeric VA1 RNA molecule was designed that contained a short antisense RNA sequence complementary to a conserved region of the HIV-1 rev encoding mRNA (28 nucleotides). This sequence, which was inserted into a projecting loop of the VA1 RNA central domain, was mainly single stranded and available for binding with its complementary sequence. The chimeric VA1 antisense was abundantly expressed in human cells constituting 3% of mRNA and promoted strong and specific inhibition of HIV-1 gene replication. The stable expression of antisense RNA in human T cells (CEM) protected these cells from HIV-1 multiplication for at least 3 months. No side effects were detected because of the lack of antisense effect upon replication of the closely related HIV-2. The VA1 gene may provide a suitably compact gene cassette for the intracellular expression of short antisense RNA directed against HIV.

Citations

Dec 12, 2001·HIV Medicine·N Dorman, A M Lever
Dec 10, 2002·Experimental Eye Research·Chooi-May LaiP Elizabeth Rakoczy
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·J BaiR Akkina
Nov 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M ScherrJ J Rossi
Jan 6, 1999·Antiviral Chemistry & Chemotherapy·M R PlayerP F Torrence

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