Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins.

Journal of Veterinary Science
Youn-Jeong LeeChang-Seon Song

Abstract

Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived ...Continue Reading

References

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Citations

Aug 3, 2011·Expert Review of Vaccines·Jimmy A Mena, Amine A Kamen
Jan 1, 2014·Journal of Virological Methods·Kang-Seuk ChoiHaan-Woo Sung
May 12, 2012·Veterinary Medicine International·Nichole L Hines, Cathy L Miller
Sep 26, 2006·Advances in Virus Research·Monique M van Oers
Apr 19, 2013·Clinical and Experimental Vaccine Research·Nak-Hyung LeeJoong-Bok Lee
Jun 19, 2019·Tropical Animal Health and Production·Sagar A KhulapeSohini Dey
Sep 4, 2018·BioMed Research International·Muhammad Bashir BelloAbdul Rahman Omar

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Methods Mentioned

BETA
ELISA

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