Protection of cultured brain endothelial cells from cytokine-induced damage by α-melanocyte stimulating hormone

PeerJ
András HarazinMiklós Vecsernyés

Abstract

The blood-brain barrier (BBB), an interface between the systemic circulation and the nervous system, can be a target of cytokines in inflammatory conditions. Pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) induce damage in brain endothelial cells and BBB dysfunction which contribute to neuronal injury. The neuroprotective effects of α-melanocyte stimulating hormone (α-MSH) were investigated in experimental models, but there are no data related to the BBB. Based on our recent study, in which α-MSH reduced barrier dysfunction in human intestinal epithelial cells induced by TNF-α and IL-1β, we hypothesized a protective effect of α-MSH on brain endothelial cells. We examined the effect of these two pro-inflammatory cytokines, and the neuropeptide α-MSH on a culture model of the BBB, primary rat brain endothelial cells co-cultured with rat brain pericytes and glial cells. We demonstrated the expression of melanocortin-1 receptor in isolated rat brain microvessels and cultured brain endothelial cells by RT-PCR and immunohistochemistry. TNF-α and IL-1β induced cell damage, measured by impedance and MTT assay, which was attenuated by α-MSH (1 and 10 pM). The peptide inhibited the cytokine-induced i...Continue Reading

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Citations

Feb 11, 2020·Fluids and Barriers of the CNS·Lilla BarnaMária A Deli
Apr 2, 2020·Fluids and Barriers of the CNS·Michelle A EricksonWilliam A Banks
Jul 17, 2020·Frontiers in Molecular Neuroscience·Lorraine SieboldStephen Ashwal
Jul 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seung-Ho SeoHong-Seok Son
Dec 16, 2020·Neural Regeneration Research·Martin A SchickWinfried Neuhaus

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Methods Mentioned

BETA
nuclear translocation
PCR
Fluorescence

Software Mentioned

MATLAB
GraphPad
ImageJ
GraphPad Prism

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