Protection of endothelial survival by peroxisome proliferator-activated receptor-delta mediated 14-3-3 upregulation

Arteriosclerosis, Thrombosis, and Vascular Biology
Jun-Yang LiouKenneth K Wu

Abstract

To determine the role of prostacyclin (PGI2) in protecting endothelial cells (ECs) from apoptosis and elucidate the protective mechanism. To evaluate the effect of PGI2 on EC survival, we treated ECs with Ad-COX1/PGIS (Ad-COPI), which augmented selectively PGI2 production or carbaprostacyclin (cPGI2) followed by H2O2 for 4 hours. Ad-COPI inhibited annexin V-positive cells and blocked caspase 3 activation. cPGI2 inhibited apoptosis in a concentration-dependent manner. L-165041 had a similar effect, suggesting the involvement of peroxisome proliferator-activated receptor-delta (PPARdelta). ECs expressed functional PPARdelta. PPARdelta overexpression enhanced whereas PPARdelta knockdown by small interfering RNA abrogated the antiapoptotic action of cPGI2 and L-165041. Our results show for the first time that PGI2 stimulated 14-3-3alpha expression via PPARdelta activation. cPGI2 and L-165041 induced binding oaf PPARdelta to PPAR response elements located between -1426 and -1477 of 14-3-3alpha promoter region, thereby activating 14-3-3alpha promoter activity and protein expression. Upregulation of 14-3-3alpha proteins resulted in an increase in Bad binding to 14-3-3alpha and a reduction in Bad translocation to mitochondria. PGI2 pro...Continue Reading

References

Jan 1, 1995·Advances in Pharmacology·K K Wu
Nov 1, 1994·The American Journal of Physiology·S K SandujaK K Wu
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B M FormanR M Evans
Sep 26, 1997·The Journal of Biological Chemistry·J ZhaS J Korsmeyer
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·X M XuK K Wu
Oct 3, 1999·Physiological Reviews·S NarumiyaF Ushikubi
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Jun 6, 2000·The Journal of Biological Chemistry·X M ZhouT Chittenden
Nov 16, 2001·The Journal of Biological Chemistry·Guri Tzivion, Joseph Avruch
Apr 3, 2002·The Journal of Biological Chemistry·Chuan-Ming HaoMatthew D Breyer
Apr 20, 2002·Science·Yan ChengGarret A FitzGerald

❮ Previous
Next ❯

Citations

Jul 20, 2011·Cardiovascular Toxicology·Maria Antonietta PanaroAngela Tesse
Feb 4, 2010·Molecular Neurobiology·Wen-Hsuan FongTeng-Nan Lin
Dec 15, 2010·Cell Death & Disease·T A YacoubianD G Standaert
Dec 9, 2008·Antioxidants & Redox Signaling·Milton HamblinY Eugene Chen
Apr 3, 2008·PPAR Research·David Bishop-Bailey
Dec 2, 2008·PPAR Research·David Bishop-Bailey, Karen E Swales
May 31, 2008·Circulation Research·Tongrong HeZvonimir S Katusic
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ke-Jie YinY Eugene Chen
Mar 19, 2009·World Journal of Gastroenterology : WJG·Jin-Bo WangTian-Xing Wu
Aug 22, 2007·PPAR Research·Rachel E NisbetC Michael Hart
Mar 28, 2009·PPAR Research·Angela TesseThierry Ragot
Jul 5, 2011·Clinical and Experimental Hypertension : CHE·Adebayo Oyekan
Nov 8, 2015·Human Molecular Genetics·Nicholas J LavalleyTalene A Yacoubian
Jan 10, 2009·PPAR Research·Nanping Wang
Aug 31, 2012·Diabetes·Xiao Yu TianYu Huang
Apr 22, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eryan FengYouping Yin
Sep 6, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Pranab Lal Pakrasi, Anil K Jain
May 7, 2015·Nuclear Receptor Signaling·Greta M P Giordano Attianese, Béatrice Desvergne
Jun 11, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Jarkko HytönenFlorian Blaschke
Oct 28, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura PiquerasDavid Bishop-Bailey
Dec 1, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Yanbo FanNanping Wang
Apr 24, 2007·Journal of Cellular Physiology·Jun-Yang LiouKenneth K Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis