Protection of extract from leaves of Ardisia compressa against benomyl-induced cytotoxicity and genotoxicity in cultured rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Marco Vinicio Ramirez-MaresE González de Mejía

Abstract

The potential of the Ardisia compressa extract (EA) was examined regarding its capacity to reduce the cytotoxic effect of benomyl on rat hepatocytes. The protective effect was evaluated by Janus Green dye exclusion method. An approximate 50% cytotoxic effect of benomyl on hepatocytes was observed at 35mug/ml after 2hr of incubation. (-)Epigallocatechin 3-gallato (EGCG) and EA decreased the viability of hepatocytes at concentrations above 3mug/ml and 2.52mug, equivalent to (+)catechin/ml, respectively. A protective effect against benomyl was observed when hepatocytes were previously exposed to EGCG (3mug/ml) or EA (2.52mug, equivalent to (+)catechin/ml) followed by incubation with benomyl (35mug/ml) alone. When EGCG or EA were in contact with cells, either simultaneously or after pretreatment with benomyl, did not protect hepatocytes. EGCG (1.3x10(-2)mug/ml) or EA (9.8x10(-2)mug, equivalent to (+)catechin/ml) inhibited 57% and 34%, respectively, the unscheduled DNA synthesis (UDS) induced by benomyl at a concentration of 23x10(-2)mum, when both were incubated with hepatocytes prior to benomyl. The simultaneous incubation of benomyl with EGCG or EA did not protect the cell against the genotoxic effect of benomyl. These results in...Continue Reading

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Citations

Sep 10, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marco Vinicio Ramírez-Mares, Elvira González de Mejía
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J I Pérez-CarreónS Villa-Treviño
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M González-AvilaS Villa-Treviño
Feb 12, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E González de MejíaS Villa-Treviño
Mar 17, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Elvira González de MejíaWenyi Wang
Oct 19, 2011·Toxicology Mechanisms and Methods·Elvira González de Mejía, Marco Vinicio Ramírez-Mares
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Reyes-LópezM de la Garza
May 1, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kara MehtapOzhan Gul

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