Protection of Fasciola hepatica in the gut mucosa of immune rats is associated with infiltrates of eosinophils, IgG1 and IgG2a antibodies around the parasites

Parasite Immunology
F J Van MilligenB A Bokhout

Abstract

We investigated the immune effector mechanisms that underlie protection against F. hepatica in the gut wall of immune rats, using (immuno)histochemistry. In the lamina propria of immune Wistar rats, four weeks after oral infection, frequencies of IgE-positive cells, eosinophils and mucosal mast cells were significantly increased, compared with naïve rats. These factors represent the traditional effector mechanisms against helminths. No significant differences were detected between the two groups in frequencies of IgM-, IgG2a-, IgG1- and IgA- positive cells, CD4- and CD8-positive cells, NK cells, macrophages, neutrophils or goblet cells. Upon challenge of immune rats with F. hepatica in an ex vivo gut segment, NEJs that migrated through the (sub)mucosa were coated with IgG1 and IgG2a antibodies and surrounded by eosinophils. No IgE or IgA antibodies were detected on the parasites. The onset of these immune effector responses, two h after challenge, was related to the expression of protection. These results suggest that NEJs are killed by an eosinophil-mediated cytotoxic response involving IgG antibodies. These antibodies were not produced in the intestine, but infiltrated the gut upon challenge. The observed immune effector resp...Continue Reading

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Citations

Jul 5, 2003·Veterinary Immunology and Immunopathology·H WedrychowiczA Płucienniczak
Apr 9, 2008·Journal of Helminthology·Marie-Hélène GuislainMarie-Lazarine Poulle
Mar 29, 2013·International Journal for Parasitology·Krisztina V VukmanSandra M O'Neill
Mar 20, 2010·FEMS Immunology and Medical Microbiology·Eleuza R MachadoLúcia H Faccioli
Oct 7, 2017·Transboundary and Emerging Diseases·N J BeesleyD J L Williams
Nov 19, 2015·Parasite Immunology·Krisztina V VukmanSandra M O'Neill
Sep 8, 2018·Parasites & Vectors·Raúl Pérez-CaballeroÁlvaro Martínez-Moreno
Oct 13, 2020·Frontiers in Immunology·Sofía FrigerioTeresa Freire
May 25, 2021·Frontiers in Cellular and Infection Microbiology·Amber DoreyRichard Lalor
Jul 3, 2021·Frontiers in Immunology·Mariela JiménezEva Salinas

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