PMID: 9166730Jun 1, 1997Paper

Protection of flupirtine on beta-amyloid-induced apoptosis in neuronal cells in vitro: prevention of amyloid-induced glutathione depletion

Journal of Neurochemistry
W E MüllerP Pialoglou

Abstract

Effective drugs are not available to protect against beta-amyloid peptide (A beta)-induced neurotoxicity. Cortical neurons from rat embryos were treated with the toxic fragment A beta25-35 at 1 microM in the presence or absence of flupirtine, a triaminopyridine, successfully applied clinically as a nonopiate analgesic drug. Five days later 1 microM A beta25-35 caused reduction of cell viability to 31.1%. Preincubation of cells with flupirtine (1 or 5 microg/ml) resulted in a significant increase of the percentage of viable cells (74.6 and 65.4%, respectively). During incubation with A beta25-35 the neurons undergo apoptosis as determined by appearance of the characteristic stepladder-like DNA fragmentation pattern and by the TUNEL technique. A beta25-35-induced DNA fragmentation could be abolished by preincubation of the cells with 1 microg/ml flupirtine. Incubation with A beta25-35 reduces the intraneuronal level of GSH from 21.4 to 7.4 nmol/10(6) cells. This depletion could be partially prevented by preincubation of the cells with flupirtine. Thus, flupirtine may be adequate for the treatment of the neuronal loss in Alzheimer's disease (where A beta accumulates in senile plaques) and probably other neurological diseases such ...Continue Reading

Citations

Jul 23, 2002·The Proceedings of the Nutrition Society·Michael GrundmanPatrick Delaney
Jun 22, 2006·Neurotoxicity Research·V Pedersen, W J Schmidt
Feb 28, 2003·Journal of Neuroscience Research·Anthony R White, Roberto Cappai
Aug 31, 2004·Journal of Neuroscience Research·Martin V PedersenVladimir Berezin
Oct 28, 1998·Environmental Health Perspectives·F J RomeroJ Romá
Oct 6, 2000·Journal of Neuroscience Research·A ThompsonC J Barrow
Apr 2, 1998·Experimental Cell Research·M Leist, P Nicotera
Jul 27, 2001·Brain Research Bulletin·D StrausakG Multhaup
Jul 13, 1999·Free Radical Biology & Medicine·F Bosch-MorellF J Romero
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Sep 16, 2000·European Journal of Pharmacology·J SeyfriedU Wüllner
Aug 11, 2004·Molecular Aspects of Medicine·Michael R Duchen
Feb 3, 2007·Brain Research Reviews·Paolo Zatta, Adrian Frank

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