Protection of GnRH analogue by chitosan-dextran sulfate nanoparticles for intravaginal application in rabbit artificial insemination

Theriogenology
L Casares-CrespoM P Viudes-de-Castro

Abstract

The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 μg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 μM and EDTA 20 mM), C5 group: 5 μg of buserelin/doe in control medium, Q4 group: 4 μg of buserelin/doe into CS-DS nanoparticles in control medium, Q5 group: 5 μg of busereline/doe into CS-DS nanoparticles in control medium). Results showed that fertility was significantly lower in C4 group compared to C5, Q5 and Q4 groups (0.7 versus 0.85, 0.85 and 0.82, respectively). On the contrary, prolificacy was similar in the four experimental groups studied (P > 0.05). We conclude that the CS-DS nanoparticles prepared by a coacervation process as carrier for buserelin acetate allows reducing the concentration of hormone used in extenders supplemented with bes...Continue Reading

Citations

May 18, 2020·Animals : an Open Access Journal From MDPI·Nesrein M Hashem, Antonio Gonzalez-Bulnes
Feb 24, 2020·Theriogenology·R M Garcia-GarciaP G Rebollar
May 1, 2021·Animals : an Open Access Journal From MDPI·María Pilar Viudes-de-CastroClara Marin
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Nesrein M Hashem, Antonio Gonzalez-Bulnes

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