Protection of human nasal respiratory epithelium from complement-mediated lysis by cell-membrane regulators of complement activation

American Journal of Respiratory Cell and Molecular Biology
S VarsanoZ Fishelson

Abstract

Complement in the respiratory tract protects the host from invading micoorganisms and other inhaled insults, but may damage normal tissue. Recently we reported that human respiratory epithelium from the nose to the alveoli expresses three cell-membrane regulators of complement activation: membrane cofactor protein (MCP, CD46), decay accelerating factor (DAF; CD55), and CD59. In this study we investigated whether two of these complement-regulatory proteins, DAF and CD59, protect human nasal epithelial cells from complement-mediated lysis. Treatment of nasal epithelial cells in suspension with 50% or 100% normal human serum (NHS) lysed small percentages of cells (8% and 16%, respectively). Addition of complement activators, rabbit serum antinasal epithelial cells (anti-NEC), or lipopolysaccharide (LPS) increased cell lysis in the presence of 50% NHS in a dose-dependent manner up to 50% and 35% lysis, respectively. Human serum deficient in C3 or C7 did not lyse nasal epithelial cells even in the presence of anti-NEC. To assay the contribution of DAF and CD59 to cell protection against lysis, nasal epithelial cells in suspension were treated with appropriate blocking antibodies. Both anti-DAF and anti-CD59 markedly increased the su...Continue Reading

Citations

Dec 21, 2010·Current Allergy and Asthma Reports·Yves LaumonnierJörg Köhl
Jun 11, 1999·Clinical and Experimental Immunology·M Närkiö-MäkeläS Meri
Apr 25, 2012·The Journal of Biological Chemistry·Oren MoskovichZvi Fishelson
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·S VarsanoJ Radnay
Jan 30, 2016·Biomarker Research·Marcio de Souza CavalcanteAna Cristina de Oliveira Monteiro-Moreira
Jun 25, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hongmei GuRagini Vittal

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