Abstract
Luteolin-7-O-glucoside (LUTG) was isolated from the plants of Dracocephalum tanguticum Maxim. Previous research has showed that LUTG pretreatment had a significant protective effect against doxorubicin (DOX)-induced cardiotoxicity by reducing intracellular calcium overload and leakage of creatine kinase and lactate dehydrogenase. But the underlying mechanisms have not been completely elucidated. In the present study, we investigated the effects of LUTG on H9c2 cell morphology, viability, apoptosis, reactive oxygen species generation, and the mitochondrial transmembrane potentials. The expression of p-PTEN, p-Akt, p-ERK, p-mTOR, and p-GSK-3β were detected by Western blotting. Compared with DOX alone treatment group, the morphological injury and apoptosis of the cells in groups treated by DOX plus LUTG were alleviated, cell viability was increased, ROS generation was lowered remarkably, and mitochondrial depolarization was mitigated. In DOX group, the expression of p-PTEN was lower than normal group and the expression of p-Akt and p-ERK was higher than normal group. In the groups treated with LUTG (20 μM), the expression of p-PTEN was upregulated and the expression of p-Akt, p-ERK, p-mTOR, and p-GSK-3β was downregulated. These re...Continue Reading
References
Mar 15, 1976·Experimental Cell Research·B W Kimes, B L Brandt
Feb 7, 2001·Circulation·S NegoroK Yamauchi-Takihara
Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Yoshiaki Taniyama, Kenneth Walsh
Nov 19, 2003·Archives of Biochemistry and Biophysics·Jean-François ThébergeAshok K Srivastava
Jul 28, 2004·Journal of Molecular and Cellular Cardiology·Gavin Y OuditPeter H Backx
Dec 30, 2004·Journal of Molecular and Cellular Cardiology·Takashi Matsui, Anthony Rosenzweig
Mar 15, 2005·The Journal of Clinical Investigation·Gerald W Dorn, Thomas Force
Sep 30, 2006·Cell Biology and Toxicology·J M Berthiaume, K B Wallace
Nov 3, 2006·Experimental & Molecular Medicine·Seon-Young KimUh-Hyun Kim
Jul 17, 2007·Neuroscience·N ShiodaK Fukunaga
Jul 31, 2007·Journal of Neurochemistry·Norifumi ShiodaKohji Fukunaga
Jul 31, 2007·Journal of Pharmacological and Toxicological Methods·Beshay N M Zordoky, Ayman O S El-Kadi
Jan 5, 2008·Circulation Research·Kyriakos N PapanicolaouKenneth Walsh
Jul 8, 2008·Basic Research in Cardiology·Hilary K SiddallMihaela M Mocanu
Sep 23, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Katsuhiko OhoriKazuaki Shimamoto
Jan 15, 2009·Acta Physiologica·B N FuglestegK Ytrehus
May 26, 2009·Cancer Chemotherapy and Pharmacology·Gangjun DuLijuan Yu
Jun 9, 2009·Journal of Natural Products·Shu-Qi WangHong-Xiang Lou
Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Yi-Wei ZhangLei Wei
May 8, 2010·International Immunopharmacology·Gangjun DuGuang Han
Jul 30, 2010·Journal of Agricultural and Food Chemistry·Wenjuan LiHui Zhang
Oct 12, 2010·Bioorganic & Medicinal Chemistry Letters·Shu-Qi WangHong-Xiang Lou
Jun 3, 2011·International Journal of Cardiology·Xiaorong HuHong Jiang
Jan 3, 2012·Archives of Pharmacal Research·Qi ZengHuizi Jin
Dec 25, 2012·Toxicology and Applied Pharmacology·M GharaneiH L Maddock
Apr 5, 2013·Journal of Biomedical Research·Danyang RenYuehua Li
Apr 30, 2013·Basic & Clinical Pharmacology & Toxicology·Ihab T Abdel-RaheemMekky M Abouzied
Jun 1, 2013·PloS One·Jing SunXiaobo Sun
Jul 23, 2013·Biochemical Pharmacology·Lamiaa A Ahmed, Shohda A El-Maraghy
Jul 25, 2013·Korean Circulation Journal·Beom Seob LeeSeok-Min Kang
Feb 14, 2014·Biochemical Pharmacology·Xue WangWei Jiang
Apr 20, 2014·BMC Cancer·Xiwei DingXiaoping Zou
Jun 3, 2014·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Eskandar AlipourAlireza Foroumadi
Sep 26, 2014·Cardiovascular Toxicology·Lucia SantacruzDanny O Jacobs
Citations
Mar 12, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Fang AnShuhua Wang
Jun 18, 2016·Oxidative Medicine and Cellular Longevity·Shreesh OjhaMohanraj Rajesh
Jun 18, 2015·Cardiovascular Toxicology·Mahdieh RahmatollahiAhmad Reza Dehpour
Mar 30, 2018·Pancreas·Qiang YuGuojian Yin
Oct 17, 2017·Bioscience Trends·Hong YaoXiuzhen Han
Feb 22, 2018·Phytotherapy Research : PTR·Zhantu LiuMing He
Dec 14, 2019·Cell Biology International·Akira TakaguriKumi Satoh
Aug 31, 2020·Journal of Biochemical and Molecular Toxicology·Meng QinLiqun Ren
Mar 24, 2018·Molecular Medicine Reports·Shenjie ChenYuangang Qiu
Apr 25, 2018·Toxicological Research·Jong-Hyun NhoJong-Choon Kim
Jun 8, 2018·International Journal of Molecular Sciences·Junli ShaoHuanwen Tang
Sep 7, 2019·Cell Biology International·Ruixin WangCuicui Li
May 22, 2020·PeerJ·YanDong ZhangFeng Wei
Jun 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei ShiGuozhen Cui
Sep 27, 2018·Inflammation·Yanan WangDaixun Jiang
Dec 5, 2018·Journal of Cellular Physiology·Xin ChenLong Chen
Mar 3, 2020·Frontiers in Physiology·Haixia XuJun Ren
Jul 18, 2020·Clinical Science·Keith DadsonFilio Billia
Oct 21, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·María D Navarro-HortalJosé L Quiles
Jul 2, 2020·Life Sciences·Meng YuanZhenbing Chen
Mar 22, 2021·Journal of Clinical Laboratory Analysis·Lina WuXiaosong Qin
Jul 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Cun LiuChanggang Sun
Apr 8, 2021·Pharmaceutical Biology·Yuan-Yuan CaoXiao-Hui Li
Oct 26, 2020·Archives of Oral Biology·Seul Ah LeeChun Sung Kim
May 17, 2021·Life Sciences·Cho-Won Kim, Kyung-Chul Choi
Aug 18, 2021·Mechanisms of Ageing and Development·Edward J CalabreseVittorio Calabrese
Sep 3, 2021·Biological & Pharmaceutical Bulletin·Suwichak Chaisit, Suree Jianmongkol