Protection of Luteolin-7-O-Glucoside Against Doxorubicin-Induced Injury Through PTEN/Akt and ERK Pathway in H9c2 Cells

Cardiovascular Toxicology
Hong YaoXiuzhen Han

Abstract

Luteolin-7-O-glucoside (LUTG) was isolated from the plants of Dracocephalum tanguticum Maxim. Previous research has showed that LUTG pretreatment had a significant protective effect against doxorubicin (DOX)-induced cardiotoxicity by reducing intracellular calcium overload and leakage of creatine kinase and lactate dehydrogenase. But the underlying mechanisms have not been completely elucidated. In the present study, we investigated the effects of LUTG on H9c2 cell morphology, viability, apoptosis, reactive oxygen species generation, and the mitochondrial transmembrane potentials. The expression of p-PTEN, p-Akt, p-ERK, p-mTOR, and p-GSK-3β were detected by Western blotting. Compared with DOX alone treatment group, the morphological injury and apoptosis of the cells in groups treated by DOX plus LUTG were alleviated, cell viability was increased, ROS generation was lowered remarkably, and mitochondrial depolarization was mitigated. In DOX group, the expression of p-PTEN was lower than normal group and the expression of p-Akt and p-ERK was higher than normal group. In the groups treated with LUTG (20 μM), the expression of p-PTEN was upregulated and the expression of p-Akt, p-ERK, p-mTOR, and p-GSK-3β was downregulated. These re...Continue Reading

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Citations

Mar 12, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Fang AnShuhua Wang
Jun 18, 2016·Oxidative Medicine and Cellular Longevity·Shreesh OjhaMohanraj Rajesh
Jun 18, 2015·Cardiovascular Toxicology·Mahdieh RahmatollahiAhmad Reza Dehpour
Aug 31, 2020·Journal of Biochemical and Molecular Toxicology·Meng QinLiqun Ren
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Sep 3, 2021·Biological & Pharmaceutical Bulletin·Suwichak Chaisit, Suree Jianmongkol

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