Protection of Mucuna pruriens seeds against Echis carinatus venom is exerted through a multiform glycoprotein whose oligosaccharide chains are functional in this role

Biochemical and Biophysical Research Communications
Roberto GuerrantiEnrico Marinello

Abstract

In a previous paper we demonstrated that extracts of Mucuna pruriens seeds (MPE) protect mice against Echis carinatus venom (EV) by an immunological mechanism. In this paper we demonstrate that the MPE immunogen generating the antibody that cross-reacts with the venom proteins is a multiform glycoprotein (gpMuc) whose immunogenic properties mainly reside in its glycan-chains. The glycoprotein was purified from the protein extract of M. pruriens seeds using Concanavalin A affinity chromatography. Using 2-D gel electrophoresis it separated into seven isoforms having MWs in the range from 20.3 to 28.7 kDa and pIs from 4.8 to 6.5. N-terminal sequencing of these spots revealed close similarity since all of them contained the consensus sequence DDREPV-DT found in soybean Kunitz-type trypsin inhibitor. We suggest that gpMuc contains both N- and O-glycans. Mild alkaline treatment but not PNGase F led to loss of reactivity, indicating that O-glycans are probably involved in the antigenicity of gpMuc.

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Citations

Sep 29, 2006·Glycoconjugate Journal·Lisa Di PatriziJohannis P Kamerling
Jan 19, 2013·Plant Foods for Human Nutrition·Lorenza BellaniSimonetta Muccifora
Jul 11, 2012·Toxicology International·Y K Gupta, S S Peshin
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May 12, 2009·Journal of Ethnopharmacology·Nget Hong TanJohn C Aguiyi
Mar 27, 2012·Phytotherapy Research : PTR·Nnadozie Stanley Hope-OnyekwereRoberto Guerranti
Mar 15, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Andrea ScirèRoberto Guerranti
Jul 19, 2018·Current Drug Discovery Technologies·Subhamay Panda, Leena Kumari
Mar 19, 2016·The Journal of Biological Chemistry·Ashish KumarDinakar M Salunke
Sep 21, 2007·International Journal of Immunopathology and Pharmacology·L TerzuoliB Porcelli

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