Protection of podocytes from hyperhomocysteinemia-induced injury by deletion of the gp91phox gene.

Free Radical Biology & Medicine
Chun ZhangPin-Lan Li

Abstract

In this study, mice lacking the gp91(phox) gene were used to address the role of NADPH oxidase in hyperhomocysteinemia-induced podocyte injury. It was found that a folate-free diet increased plasma homocysteine levels, but failed to increase O(2)(-) production in the glomeruli from gp91(phox) gene knockout (gp91(-/-)) mice, compared with wild-type (gp91(+/+)) mice. Proteinuria and glomerular damage index (GDI) were significantly lower, whereas the glomerular filtration rate (GFR) was higher in gp91(-/-) than in gp91(+/+) mice when they were on the folate-free diet (urine albumin excretion, 21.23+/-1.88 vs 32.86+/-4.03 microg/24 h; GDI, 1.17+/-0.18 vs 2.59+/-0.49; and GFR, 53.01+/-4.69 vs 40.98+/-1.44 microl/min). Hyperhomocysteinemia-induced decrease in nephrin expression and increase in desmin expression in gp91(+/+) mice were not observed in gp91(-/-) mice. Morphologically, foot process effacement and podocyte loss due to hyperhomocysteinemia were significantly attenuated in gp91(-/-) mice. In in vitro studies of podocytes, homocysteine was found to increase gp91(phox) expression and O2(*)(-) generation, which was substantially inhibited by gp91(phox) siRNA. Functionally, homocysteine-induced decrease in vascular endothelial ...Continue Reading

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Citations

Mar 27, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Caixia LiPin-Lan Li
Jun 8, 2011·Pflügers Archiv : European journal of physiology·Chun ZhangPin-Lan Li
Jun 15, 2010·The Journal of Pharmacology and Experimental Therapeutics·Krishna M BoiniPin-Lan Li
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Cai-Xia LiPin-Lan Li
Jul 25, 2012·Free Radical Biology & Medicine·Qiaojing YanJian Yao
Dec 10, 2013·BioMed Research International·Shan ChenChun Zhang
Feb 16, 2016·The Journal of Pathology·Changbin LiRujun Gong
Oct 21, 2014·Antioxidants & Redox Signaling·Justine M AbaisPin-Lan Li
Sep 7, 2011·The American Journal of Pathology·Krishna M BoiniPin-Lan Li
Sep 30, 2014·Cellular & Molecular Immunology·Arvind PandaySanjay Batra
Sep 7, 2016·Oxidative Medicine and Cellular Longevity·Cheng WanChun Zhang
Nov 11, 2017·Antioxidants & Redox Signaling·Owais M BhatPin-Lan Li
Sep 17, 2013·Journal of Cellular Physiology·Marc AndersonStuart E Dryer
Feb 5, 2015·American Journal of Physiology. Renal Physiology·Hua SuChun Zhang
Dec 21, 2010·Cell Biology International·Fang-Fang HeXian-Fang Meng
Oct 29, 2010·American Journal of Physiology. Renal Physiology·Sun-Young HwangKarmin O
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Feb 7, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Haiyan XuXueyuan He
Jun 3, 2020·American Journal of Hypertension·Ning GaoRui Xu

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