Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish

International Journal of Nanomedicine
Abraham Heriberto Garcia CampoyAlethia Muñiz Ramirez

Abstract

The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus. EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques. Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased. The synthesized EP/AgNPs showed an absorption peak at 413 nm on ultraviolet-visible spectroscopy, revealing the surface plasmon resonance of the nanoparticles. Transmission electron microscopy indicated that most of the particles were spherical, with a diameter of 10-12 nm, a polydispersity index of 0.197, and a zeta potential of -32.25 mV, suggesting high stability of the nanoparticles. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury. The results indicate that EP/AgNPs have good antidiabetic activity and therefor...Continue Reading

Citations

Dec 14, 2018·Frontiers in Endocrinology·Ayushi PriyamShweta Gehlout
Sep 4, 2020·Plants·Abraham Garcia-CampoyAlethia Muñiz-Ramirez
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ema BurlacuLavinia Berta
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Zeinab NouriMohammad Abdollahi
Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Fang-Jun LinRen-You Gan
Jul 7, 2021·Journal of Biomaterials Science. Polymer Edition·Rakesh Kumar PaulKaisar Raza
Aug 8, 2021·Nanomaterials·Alba Pensado-LópezFernando Torres Andón

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Methods Mentioned

BETA
surface plasmon resonance
infrared spectroscopy
transmission electron microscopy
dynamic light scattering
enzyme-linked immunoassay
ELISA

Software Mentioned

GraphPad Prism

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