Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis

Thrombosis Research
Toshiaki IbaIsao Nagaoka

Abstract

Injury and loss of the endothelial glycocalyx occur during the early phase of sepsis. We previously showed that antithrombin has a protective effect on this structure in vitro. Here, we investigated the possible protective effects of antithrombin in an animal model of sepsis. Wistar rats were injected with endotoxin, and circulating levels of syndecan-1, hyaluronan, albumin, lactate and other biomarkers were measured in an antithrombin-treated group and an untreated control group (n = 6 in each group). Intravital microscopy was used to observe leukocyte adhesion, microcirculation, and syndecan-1 staining. The circulating levels of syndecan-1 and hyaluronan were significantly reduced in the antithrombin-treated group, compared with the untreated controls. Lactate levels and albumin reduction were significantly attenuated in the antithrombin-treated group. Intravital microscopic observation revealed that both leukocyte adhesion and blood flow were better maintained in the treatment group. The syndecan-1 lining was disrupted after endotoxin treatment, and this derangement was attenuated by treatment with antithrombin. Antithrombin effectively maintained microcirculation and vascular integrity by protecting the glycocalyx in a rat ...Continue Reading

Citations

Sep 3, 2019·Frontiers in Neurology·Şefik Evren Erdener, Turgay Dalkara
Jan 12, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Dong ZhangXiao-Zhi Wang
Aug 14, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Hideshi OkadaHiroyuki Tomita
Feb 23, 2020·Journal of Intensive Care·Nobuyuki YokoyamaTakahisa Goto
Sep 13, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Toshiaki IbaJerrold H Levy
May 28, 2019·Journal of Clinical Medicine·Toshiaki IbaTheodore E Warkentin
Aug 29, 2019·Frontiers in Pediatrics·Carlo PietrasantaOfer Levy
Feb 10, 2020·The American Journal of Pathology·Sophie Dogné, Bruno Flamion
Feb 10, 2020·The American Journal of Pathology·Michael S Goligorsky, Dong Sun
Apr 13, 2021·Clinical Hemorheology and Microcirculation·David AstapenkoVladimir Cerny
Jun 12, 2021·The American Journal of Pathology·Haruka OkamotoShinji Ogura
Feb 20, 2021·The Journal of Trauma and Acute Care Surgery·Mohamed ElSaadaniJose L Pascual
Jul 22, 2021·International Journal of Laboratory Hematology·Jerrold H LevyJean M Connors
Aug 28, 2021·International Journal of Molecular Sciences·Kazuhiro Moriyama, Osamu Nishida

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