Protection of tubular epithelial cells during renal injury via post-transcriptional control of BMP7

Molecular and Cellular Biochemistry
Jiping SunJing Lv

Abstract

Severe injury of renal tubular epithelial cells may cause acute renal failure, the progression of which results in renal fibrosis, and obstructive nephropathy. Transforming growth factor β 1 and bone morphogenic protein 7 (BMP7) play contradicting roles in and coordinate the process of epithelial-to-mesenchymal transition of renal tubular epithelial cells, but the molecular regulation of BMP7 remains ill-defined. Here, we addressed this question. We found that after induction of unilateral ureteral obstruction (UUO) in mice, the increases in BMP7 mRNA were much more pronounced than BMP7 protein in kidney, suggesting the presence of post-transcriptional control of BMP7. Moreover, significant increases in a BMP7-targeting microRNA, miR-384-5p, were detected in the mouse kidney post UUO. Overexpression of miR-384-5p significantly decreased BMP7 protein, while depletion of miR-384-5p significantly increased BMP7 protein in renal epithelial cells. Bioinformatics study showed that miR-384-5p appeared to suppress BMP7 protein translation, through its direct binding to the 3'-UTR of BMP7 mRNA. Furthermore, suppression of miR-384-5p in vivo attenuated severity of renal injury by UUO. Together, our study sheds light on miR-384-5p as a cr...Continue Reading

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Citations

May 23, 2019·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Laurence M BlackAnupam Agarwal
Sep 26, 2019·RNA Biology·Youling FanJun Zhou

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Methods Mentioned

BETA
flow cytometry
transfection
fluorescence-activated cell sorting
FACS

Software Mentioned

GraphPad Prism
NIH ImageJ

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