Protection of Xenopus laevis embryos against alcohol-induced delayed gut maturation and growth retardation by peroxiredoxin 5 and catalase

Journal of Molecular Biology
Ying PengMarie C Lin

Abstract

Accumulated evidence indicates that maternal alcohol consumption causes fetal enteric damage and growth retardation. In this study, we investigated the underlying molecular mechanisms in a Xenopus model of fetal alcohol exposure. We established a condition of transient alcohol exposure that produces tadpoles with delayed gut maturation and decreased body length. We then investigated the roles of reactive oxygen species (ROS) and reactive nitrogen species (RNS) by microinjecting plasmids expressing catalase and peroxiredoxin 5 (PRDX5) into two-cell stage embryos. Finally, the effects of these enzymes on the expression of key gut developmental genes were determined by animal cap explant assay. We showed that exposure of Xenopus embryos to 0.5% alcohol from stage 13 to stage 22 produced tadpoles with delayed gut maturation, reduced growth, and down-regulation in several gut developmental genes, with VegT, Pax6 and Sox17 most vulnerable. We further demonstrated that microinjection of catalase attenuated alcohol-induced ROS production and restored the expression of VegT and Pax6, but protected the embryos from delayed gut development and retarded growth only partially. By contrast, microinjection of PRDX5 reduced both ROS and RNS pr...Continue Reading

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Citations

Jul 18, 2012·Environmental Science and Pollution Research International·Abbas GüngördüMurat Ozmen
Oct 28, 2010·Antioxidants & Redox Signaling·Bernard KnoopsJean-Paul Declercq
Sep 12, 2013·PloS One·Valérie Van der EeckenBernard Knoops
Jun 30, 2012·International Journal of Proteomics·Stephen MasonFeng C Zhou
Jul 10, 2007·American Journal of Obstetrics and Gynecology·Ridgely Fisk Green, Joan Marilyn Stoler
Oct 27, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Abraham Fainsod, Hadas Kot-Leibovich
Feb 10, 2018·BMC Evolutionary Biology·Marc PirsonBernard Knoops
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Jul 28, 2010·Alcoholism, Clinical and Experimental Research·Ling LongYing Peng
Jul 21, 2020·Bioelectricity·Vaibhav P Pai, Dany Spencer Adams

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