Protective action of a modified fragment of galanine in rats with doxorubicin-induced heart failure

Biomedit︠s︡inskai︠a︡ khimii︠a︡
I M StudnevaO I Pisarenko

Abstract

The use of the anticancer drug doxorubicin (Dox) is limited due to its cardiotoxic effect. Using the method of automatic solid-phase peptide synthesis, we obtained a synthetic agonist of galanin receptors GalR1-3 [RAla14, His15]-galanine (2-15) (G), exhibiting cardioprotective properties. It was purified by high performance liquid chromatography (HPLC). The homogeneity and structure of the peptide was confirmed by HPLC, 1H-NMR spectroscopy and mass spectroscopy. The purpose of this study was to study the effect of G on the metabolism and cardiac function of rats with chronic heart failure (CHF) caused by Dox. Experiments were performed using male Wistar rats weighing 280-300 g. The control group of animals (C) was intraperitoneally treated with saline for 8 weeks; the doxorubicin group (D) of rats was intraperitoneally treated with Doх; the group of Doх + peptide G (D+G) received intraperitoneally injections of Doх and subcutaneously injections of peptide G; the peptide G group (G) was subcutaneously treated with G. At the beginning and at the end of the study, the concentration of thiobarbituric acid reactive substances (TBARS) and the activity of creatine kinase-MB (CK-MB) were determined in blood plasma; the animals were wei...Continue Reading

References

Jan 7, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M M Sayed-AhmedM M El-Merzabani
Mar 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Malgorzata Tokarska-SchlattnerUwe Schlattner
Jun 10, 2008·Nature Protocols·Andrey V KuznetsovWolfram S Kunz
Apr 3, 2012·Journal of Molecular and Cellular Cardiology·Yanti OctaviaAn L Moens
Apr 12, 2014·Circulation Research·Craig A Lygate, Stefan Neubauer
Jul 24, 2015·Mini Reviews in Medicinal Chemistry·Sevasti ZervouCraig A Lygate
May 8, 2018·Peptides·Larisa SerebryakovaOleg Pisarenko

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Citations

Jul 2, 2019·Biomedit︠s︡inskai︠a︡ khimii︠a︡·L I SerebryakovaO I Pisarenko
Feb 11, 2020·Frontiers in Cardiovascular Medicine·Anais AudebrandCanan G Nebigil

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