Protective activity of Streptococcus pneumoniae Spr1875 protein fragments identified using a phage displayed genomic library.

PloS One
Angela CardaciConcetta Beninati

Abstract

There is considerable interest in pneumococcal protein antigens capable of inducing serotype-independent immunoprotection and of improving, thereby, existing vaccines. We report here on the immunogenic properties of a novel surface antigen encoded by ORF spr1875 in the R6 strain genome. An antigenic fragment encoded by spr1875, designated R4, was identified using a Streptococcus pneumoniae phage displayed genomic library after selection with a human convalescent serum. Immunofluorescence analysis with anti-R4 antisera showed that Spr1875 was expressed on the surface of strains belonging to different serotypes. Moreover, the gene was present with little sequence variability in 27 different pneumococcal strains isolated worldwide. A mutant lacking Spr1875 was considerably less virulent than the wild type D39 strain in an intravenous mouse model of infection. Moreover, immunization with the R4 recombinant fragment, but not with the whole Spr1875 protein, induced significant protection against sepsis in mice. Lack of protection after immunization with the whole protein was related to the presence of immunodominant, non-protective epitopes located outside of the R4 fragment. In conclusion, our data indicate that Spr1875 has a role i...Continue Reading

References

Apr 10, 1999·Journal of Bacteriology·F IannelliG Pozzi
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Jun 18, 2002·Research in Microbiology·Barbara J PearceGianni Pozzi
Mar 14, 2003·International Journal for Parasitology·Elisa BeghettoNicola Gargano
Dec 20, 2003·Lancet·Thomas M File
May 20, 2004·Vaccine·D BogaertR de Groot
Dec 13, 2005·Current Opinion in Microbiology·Sven Hammerschmidt
Dec 15, 2007·Cell Host & Microbe·Margaretha Stålhammar-CarlemalmGunnar Lindahl
Apr 24, 2008·Molecular Microbiology·Girbe BuistOscar P Kuipers
Nov 27, 2009·Postgraduate Medicine·Susan J RehmWilliam Schaffner

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Citations

Oct 3, 2013·PloS One·Salvatore PapasergiConcetta Beninati
Jul 31, 2013·Critical Reviews in Microbiology·Michelle DarrieuxLuciana Cezar de Cerqueira Leite
Apr 17, 2013·Microbial Pathogenesis·Samuele PeppoloniElisabetta Blasi
Jun 7, 2014·The Journal of Biological Chemistry·Marco BuscettaConcetta Beninati
Dec 20, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Germana LentiniAngelina Midiri
Aug 8, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Germana LentiniConcetta Beninati

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Methods Mentioned

BETA
phage display
flow cytometry
ELISA
PCR

Software Mentioned

ClustalW
CellQuest
SignalP

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