Protective antigenic sites in respiratory syncytial virus G attachment protein outside the central conserved and cysteine noose domains

PLoS Pathogens
Jeehyun LeeSurender Khurana

Abstract

Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract disease in infants. Previously, we elucidated the antibody repertoire following primary RSV infection in infants. Whole genome-fragment phage display libraries (GFPDL) expressing linear and conformational epitopes from RSV bound 100-fold more phages within attachment protein (G) following primary RSV infection. The G-reactive epitopes spanned the N- and C-termini of G ectodomain, in addition to the central conserved domain (CCD). In the current study, we examined the contribution of antigenic regions of G outside of the CCD to RSV-specific immunity. We evaluated the immunogenicity, neutralization and protective efficacy of all RSV-G antigenic sites identified following primary RSV infection using recombinant E. coli expressed G ectodomain (REG), CCD-deleted G ectodomain (REG ΔCCD), N- and C-terminal G subdomains, and antigenic site peptides. The REG ΔCCD, N- and C-terminal subdomains and peptides generated antibody titers in rabbits and mice that bound fully glycosylated Recombinant Mammalian expressed G ectodomain (RMG) and intact RSV virion particles but minimal in vitro neutralization titers compared with the intact G ectodomain. Vaccinated mice ...Continue Reading

References

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Mar 11, 2018·Science Immunology·Stanislav O FedechkinRebecca M DuBois

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Citations

Feb 8, 2020·The Journal of Infectious Diseases·Fabio MidullaAlessandra Pierangeli
Apr 9, 2020·Expert Opinion on Biological Therapy·Seyhan Boyoglu-Barnum, Ralph A Tripp
Dec 20, 2019·Journal of Virology·Stanislav O FedechkinRebecca M DuBois
Aug 14, 2019·Frontiers in Immunology·Seyhan Boyoglu-BarnumLarry J Anderson
Jul 11, 2021·Vaccine·Rama RaghunandanNancy Hosken
Aug 11, 2021·Viruses·Larry J AndersonSuxiang Tong
Jul 2, 2020·Cell Reports·Ponraj Prabakaran, Partha S Chowdhury

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Methods Mentioned

BETA
phage display
gel filtration
ELISA
chip
glycosylation
PCR
surface
biosensor

Software Mentioned

BioEdit
GFPDL
LivingImage
Bio
Rad ProteOn Manager
Living Image

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