Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide.

BioMed Research International
Xiaonan ZhouLing Kui

Abstract

Lonicera japonica is a traditional Chinese herbal medicine with antioxidation, anti-inflammatory, antibacterial, and immunoregulation functions. A method to isolate polysaccharides from Lonicera japonica (LJP) has been reported previously by our group. We also reported previously that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides. In this study, we investigated the protective effect of LJP on cardiomyocytes of mice injured by hydrogen peroxide (H2O2). The results showed that LJP can increase the cardiomyocyte viability and the activities of the enzyme (SOD, CAT, GSH-Px, AST, CPK, and LDH) in cardiomyocytes of mice injured by hydrogen peroxide. The results of intracellular ROS contents showed that a high dose (40 μg mL-1) of LJP had the best effects on protecting the cardiomyocytes of mice injured by H2O2. In addition, the measurement results of the cardiomyocyte apoptosis and the activity of caspase-3, caspase-8, and caspase-9 in cardiomyocytes confirmed this conclusion from another perspective.

References

Jun 15, 1994·Biochemical and Biophysical Research Communications·J B PrinsD P Cameron
Sep 23, 1997·Journal of Molecular and Cellular Cardiology·E GiladC Szabó
Oct 28, 2003·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Goro AsanoYuhichi Sugisaki
Aug 30, 2008·Journal of Cellular and Molecular Medicine·Elisabetta BorchiChiara Nediani
Apr 30, 2010·International Journal of Molecular Medicine·Tzung-Yan LeeHan-Chieh Lin
Aug 31, 2013·Journal of Molecular and Cellular Cardiology·Masayoshi OikawaChen Yan
Jun 14, 2014·Biochemical and Biophysical Research Communications·Yixia ZhaoJian Zhang
Jul 26, 2014·Journal of the American College of Cardiology·Javier Sanz, George Dangas
Apr 18, 2015·Drug and Chemical Toxicology·Nathalie ChahineRamez Chahine
Dec 2, 2015·Bioorganic & Medicinal Chemistry Letters·Bin LiuShiming Liu
Jan 19, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Nut Koonrungsesomboon, Juntra Karbwang
Feb 9, 2016·Journal of Molecular and Cellular Cardiology·Yun LiuYong-Yuan Guan
Feb 24, 2016·Biological Trace Element Research·Jiayong TangHua Zhao
Apr 14, 2016·Canadian Journal of Physiology and Pharmacology·Huang HuangJichun Liu
Sep 8, 2016·European Journal of Preventive Cardiology·Massimo F PiepoliAntonio Pelliccia
Nov 5, 2016·Biochimica Et Biophysica Acta·Maureen Redza-Dutordoir, Diana A Averill-Bates
Apr 6, 2017·Current Atherosclerosis Reports·Mahmoud I TrainaE Murat Tuzcu
Jun 8, 2017·International Journal of Biological Macromolecules·Jing-Yi ZhangJing-Jing Liu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
MDA
flow cytometry

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis