Protective Effect of Ad-VEGF-Bone Mesenchymal Stem Cells on Cerebral Infarction

Turkish Neurosurgery
Bo ChenQing Lan

Abstract

To understand the mechanism of intracerebroventricular transplantation of vascular endothelial growth factor (VEGF) genemodified bone mesenchymal stem cells (BMSCs) in rats after cerebral infarction. The middle cerebral artery occlusion ischemia/reperfusion (MCAO I/R) model was established in rats using the Zea-Longa suture method. A recombinant adenovirus (Ad-VEGF) was engineered to express VEGF. The rats were divided into 3 groups. Control BMSC infected with control adenovirus (BMSC-Ad), BMSC infected by Ad-VEGF (BMSC-Ad-VEGF), and phosphate buffered saline (PBS) suspension were injected into the intracerebroventricular system of the rats in groups 1, 2 and 3 respectively, 24 hours after middle cerebral artery occlusion (MCAO). The neurological function of rats was evaluated with the modified Neurological Severity Scores (mNSS). The infarct volume of brain in rats was determined using 2,3,5-triphenyltetrazolium chloride (TTC) stain at 14 days. GFAP and pGSK3β expression of ischemic penumbra was determined using immunohistochemical method. GFAP, pAKT, AKT, and pGSK3β expressions were determined with Western blot. Functional improvement was accelerated in animals receiving BMSC-Ad, while improvement at all times between 7 days ...Continue Reading

Citations

Jun 3, 2016·Frontiers in Molecular Neuroscience·Amanda J Craig, Gary D Housley
Jun 1, 2018·Neurologia Medico-chirurgica·Yoichi YoshidaYasuo Iwadate
Mar 3, 2018·Frontiers in Pharmacology·Mikhail E SokolovRustem R Islamov
Jul 3, 2021·Pharmaceutics·Noha AttiaJose Luis Pedraz

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