Protective effect of Berberine pretreatment in hepatic ischemia/reperfusion injury of rat

Transplantation Proceedings
M ShengH Du

Abstract

Berberine (BBR) has been demonstrated to protect against hepatic ischemia/reperfusion (I/R) injury. However, the exact mechanism is largely unknown. In the present study, we examined the role of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and mammalian target of rapamycin (mTOR) in the protective effect of BBR on hepatic I/R-mediated apoptosis in rats. Adult male Sprague-Dawley rats were assigned randomly to groups of sham, ischemia/reperfusion (I/R), I/R+DMSO (vehicle) and I/R+BBR (100 mg/kg/d, 2 weeks). The hepatic cold ischemia model was established by perfusing the liver with heparinized cold saline through the portal vein for 20 minutes. The liver function and oxidative stress level were measured by biochemical and histopathologic examinations. The apoptotic rate was determined by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay. The expression of Bcl-2, Bax, caspase-3, and the phosphorylation of Akt and mTOR were assayed by Western blot analysis and immunohistochemistry. Compared with the I/R group, BBR dramatically attenuated the histopathologic damage, restored the liver function, and decreased the oxidative stress level. Simultaneously, BBR significantly ameliorated apopto...Continue Reading

References

Jan 1, 1981·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J Limmer, R Y Calne
Jun 1, 2002·Science·Lewis C Cantley
Jul 26, 2011·American Journal of Surgery·Maximilian von HeesenMohammed Reza Moussavian
Sep 3, 2011·Journal of Cellular and Molecular Medicine·A FaggianoG Vitale
Nov 4, 2011·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yavuz AlbayrakBunyami Unal
Jun 8, 2012·Current Cancer Drug Targets·Marco RossiMichele Caraglia
Jul 28, 2012·The Journal of Surgical Research·Xiaohua YangHaixin Qian
Oct 13, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N I NativM L Yarmush
Dec 13, 2012·Expert Opinion on Therapeutic Patents·Inder Pal Singh, Shivani Mahajan
Jun 29, 2013·Brain Research·Fatemeh Zare MehrjerdiHamidreza Pazoki-Toroudi
Nov 2, 2013·European Journal of Pharmacology·Dhirendra Pratap Singh, Kanwaljit Chopra
Jan 12, 2013·Journal of Liver: Disease & Transplantation·Kimberly J RiehleJean S Campbell
Mar 26, 2014·Apoptosis : an International Journal on Programmed Cell Death·Keke ChenFeng Gao

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Citations

Oct 18, 2015·Experimental Eye Research·Delu SongJoshua L Dunaief
Jul 28, 2016·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Stephen M CovingtonLuis H Toledo-Pereyra
Mar 31, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ziru LiWeizhen Zhang
Oct 14, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Esam O KamelFares E M Ali
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Tao ZhangJiliang Wang
Dec 15, 2018·Journal of Cellular and Molecular Medicine·Wan-Jung LuRay-Jade Chen
Aug 11, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dai-Qiang LiuDa-Wei Ye
Sep 20, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Suhair A AbdellatiefShefaa A M El-Mandrawy

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