Protective effect of BMSCs-derived exosomes mediated by BDNF on TBI via miR-216a-5p

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Huiyou XuXuyi Chen

Abstract

BACKGROUND Transplantation of exosomes derived from mesenchymal stem cells (MSCs-Exo) can improve the recovery of neurological function in rats after traumatic brain injury (TBI). We tested a new hypothesis that BDNF-mediated MSCs-Exo could effectively promote functional recovery and neurogenesis of rats after TBI. MATERIAL AND METHOD BMSCs of rats were extracted by whole bone marrow culture, BDNF was added to BMSCs for intervention, supernatant was collected, and exosomes were separated and purified by hypercentrifugation. Exosomes were identified by WB, TEM and particle size analysis and subsequently used in cell and animal experiments. We investigated the recovery of sensorimotor function and spatial learning ability, inflammation inhibition and neuron regeneration in rats after TBI. RESULTS Compared with group MSCs-Exo, group BDNF-mediated MSCs-Exo showed better effects in promoting the recovery of sensorimotor function and spatial learning ability. BDNF-mediated MSCs-Exo successfully inhibited inflammation and promoted neuronal regeneration in vivo and in vitro. We further analyzed miRNA in BDNF-mediated MSCs-Exo and MSCs-Exo, and found that the expression of miR-216a-5p in BDNF-mediated MSCs-Exo was significantly higher t...Continue Reading

Citations

Jun 18, 2020·Biomolecules·Maria Antonietta PanaroChiara Porro
Aug 9, 2020·Frontiers in Neurology·Vivian A GuedesJessica M Gill
Mar 9, 2021·Stem Cells International·Qian JinWenrong Xu

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Methods Mentioned

BETA
transfection
protein assay
electrophoresis
transmission electron microscopy

Software Mentioned

Excel
SPSS
Ethovision

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