Protective Effect of Carvacrol on Oxidative Stress and Cellular DNA Damage Induced by UVB Irradiation in Human Peripheral Lymphocytes

Journal of Biochemical and Molecular Toxicology
Balakrishnan AristatileKodukkur Viswanathan Pugalendi

Abstract

Exposure to ultraviolet B (UVB; 280-320 nm) radiation induces the formation of reactive oxygen species (ROS) in the biological system. In this study, we examined the protective effect of carvacrol on UVB-induced lipid peroxidation and oxidative DNA damage with reference to alterations in cellular an-tioxidant status in human lymphocytes. A series of in vitro assays (hydroxyl radical, superoxide, nitric oxide, DPPH (2,2-Diphenyl-1-picryl hydrazyl), and ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assays) demonstrate antioxidant property of carvacrol in our study. UVB exposure significantly increased thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LHPs), % tail DNA and tail moment; decreased % cell viability and antioxidant status in UVB-irradiated lymphocytes. Treatment with carvacrol 30 min prior to UVB-exposure resulted in a significant decline of TBARS, LHP, % tail DNA, and tail moment and increased % cell viability as carvacrol concentration increased. UVB irradiated lymphocytes with carvacrol alone (at 10 μg/mL) gave no significant change in cell viability, TBARS, LHP, % tail DNA, and tail moment when compared with normal lymphocytes. On the basis of our results, we c...Continue Reading

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Citations

May 24, 2011·Journal of Natural Medicines·Balakrishnan AristatileKodukkur Viswanathan Pugalendi
Sep 18, 2014·Journal of Medicinal Food·Karine Rech BegniniFrancisco Augusto Burkert Del-Pino
Feb 24, 2016·Biochemistry Research International·Saeed SamarghandianAbasalt Borji
Apr 26, 2016·Journal of Biochemical and Molecular Toxicology·Emrah Hicazi AksuAli Doğan Ömür
Jul 1, 2020·International Journal of Molecular Sciences·Ekaterina ProshkinaAlexey Moskalev
Oct 11, 2017·Mutagenesis·Malgorzata M DobrzynskaKatarzyna Owczarska
May 26, 2018·Archives of Physiology and Biochemistry·Mehmet GüvençŞule Yurdagül Özsoy
Jan 1, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hamed ShooreiMajid Shokoohi
May 11, 2021·CNS & Neurological Disorders Drug Targets·Mohammad Yassin ZamanianGholamreza Bazmandegan

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