PMID: 9637395Jun 24, 1998Paper

Protective effect of deferoxamine on sodium nitroprusside-induced apoptosis in PC12 cells

Neuroscience Letters
M S DesoleE Miele

Abstract

Reportedly, the generation of nitric oxide (NO) may lead to iron mobilization from ferritin disrupting intracellular iron homeostasis and increasing levels of reactive oxygen species. In the present study, we evaluated the role of endogenous iron in NO-induced apoptosis in PC12 cells. Apoptosis was tested by flow cytometry, fluorescence microscopy and terminal deoxynucleotidyl transferase-mediated 2'-deoxy-uridine 5'-triphosphate nick end labeling (TUNEL) technique. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When incubated with 0.5-0.75 mM sodium nitroprusside (SNP, a chemical NO donor), PC12 cells were shown to undergo apoptosis. In addition, SNP induced a time-dependent decrease in cell viability. Since deferoxamine (0.05-0.1 mM), a powerful iron chelator, inhibited both SNP-induced apoptosis and the decrease in cell viability, we suggest that these NO effects may be dependent upon iron mobilization within the cell.

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J R Lancaster, J B Hibbs
Dec 1, 1990·Archives of Biochemistry and Biophysics·D W Reif, R D Simmons
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L J Ignarro
Mar 19, 1988·Lancet·J Martin, S Moncada
Aug 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M Stefanovic-RacicC H Evans
Aug 1, 1996·Neurochemistry International·V L Dawson, T M Dawson

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Citations

Oct 19, 2002·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Petr DobsákM Horky
Aug 23, 2001·International Immunopharmacology·P K KimT R Billiar
Jan 14, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W Paschen, J Doutheil
Jun 22, 2002·Annals of the New York Academy of Sciences·Peter K M KimYoung-Myeong Kim
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Key-Sun Kim
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Ki-Mo KimYoung-Myeong Kim
Jun 27, 2009·European Journal of Pharmacology·Wei BiLanrong Bi
Feb 24, 2005·Brain Research·Karine LortiePaul Morley
Mar 23, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Manish M TiwariPhilip R Mayeux
Jun 25, 2005·Journal of Medicinal Chemistry·Ming ZhaoShiqi Peng

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis