Protective effect of down-regulated microRNA-27a mediating high thoracic epidural block on myocardial ischemia-reperfusion injury in mice through regulating ABCA1 and NF-κB signaling pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jin-Yu LiuZhi-Yong Gao

Abstract

Myocardial ischemia-reperfusion injury (MI/RI) is linked with serious inflammatory response, which may lead to myocyte injury. The important role of miR-27a in MI/RI has been previously demonstrated. Therefore, this study aims to investigate the effect of miR-27a targeting ABCA1 on MI/RI by investigating its influences on high thoracic epidural block (HTEB) mediated by the NF-κB signaling pathway. A MI/RI mouse model and a MI/RI with HTEB mouse model were established to observed the histopathological changes and ultrastructure of myocardial tissues and assess the positive expression of ABCA1. Cardiac troponin T (cTnT), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were determined using enzyme-linked immunosorbent assay (ELISA). The expression of miR-27a, ABCA1, IκBα and p65 in myocardial cells that transfected with different mimic, inhibitor and siRNAs was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot, with cell apoptosis analyzed by flow cytometry. ABCA1 was a target gene of miR-27a and was lowly expressed in myocardial tissues of MI/RI mice. The decreased content of cTnT, TNF-α and IL-1β and expression of miR-27a and p65 as well as increased expression of AB...Continue Reading

References

Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Mar 27, 2001·Current Opinion in Lipidology·G Schmitz, T Langmann
Apr 10, 2003·The Annals of Thoracic Surgery·Tayfun AybekAnton Moritz
Apr 18, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·David Evans, F Ulrich Beil
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marie-Christine Gerbod-GiannoneAlan R Tall
Oct 15, 2008·International Reviews of Immunology·Fazlul H SarkarDejuan Kong
Sep 29, 2009·Plant, Cell & Environment·Alejandra A Covarrubias, José L Reyes
Jan 12, 2010·Biochemical and Biophysical Research Communications·Sang Yun KimJae Bum Kim
Mar 1, 2012·Seminars in Cardiothoracic and Vascular Anesthesia·Anja FrankTobias Eckle
Mar 16, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Simon-Pierre GuayLuigi Bouchard
Aug 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Weidong QianZhensheng Wang
Sep 17, 2014·Biochemical and Biophysical Research Communications·Yan WangChao-Ke Tang
Oct 4, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xuan LiGuonian Wang
Feb 17, 2015·World Journal of Critical Care Medicine·Antonio SiniscalchiStefano Faenza
May 1, 2015·Journal of Cellular Biochemistry·Chun-Li SongZhi-Xian Liu
Sep 16, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Rong Rong LiuLi Hua Chen
Oct 29, 2015·BioMed Research International·Tingyang ZhouLi Zuo
Apr 9, 2016·Cells·Amanda L Rinkenbaugh, Albert S Baldwin
Jan 20, 2017·Experimental and Therapeutic Medicine·Xianchi LiPeiying Zhang

❮ Previous
Next ❯

Citations

Oct 9, 2020·Immunological Investigations·Fatemeh Sabet Sarvestani, Negar Azarpira
Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shun ZhangWujun Chen
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Aug 21, 2021·Oxidative Medicine and Cellular Longevity·Yandong BaoDalin Jia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis