Protective effect of edaravone against manganese-induced toxicity in cultured rat astrocytes

Environmental Toxicology and Pharmacology
Vedat EvrenDilek Taskiran

Abstract

Manganese (Mn), a trace metal, is essential for maintaining the normal regulation of many biochemical and cellular processes. However, accumulation of Mn due to excessive environmental exposure leads to neurological impairment, referred to as manganism. Edaravone (EDA) is a potent free radical scavenger that has been clinically shown to reduce the neuronal injury after cerebral ischemia. In the present study, we aimed to examine the protective effects of EDA against Mn toxicity in astrocyte cultures. Astrocyte cultures were prepared from cerebral cortices of newborn Sprague-Dawley rats. The experiments were performed between 16 and 18 days of cultures. Astrocytes were treated in DMEM medium containing Mn (1-1000μM) for 24h to test Mn toxicity. In order to assess the effect of EDA, cells were pre-treated with different doses of EDA (10, 100 and 1000μM) 6h before Mn treatment. Cell viability (MTT), apoptotic cell death (Hoechst test) and lipid peroxide levels were evaluated in cultures. Our results showed that Mn significantly and dose-dependently reduced cell viability in astrocyte cultures. The apoptotic cell death and lipid peroxides were significantly higher in Mn treated cultures. Treatment of astrocytes with EDA successfull...Continue Reading

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Citations

Jan 19, 2016·Journal of Biochemical and Molecular Toxicology·Melda ApaydinDilek Taskiran
Aug 5, 2018·Journal of Neuroinflammation·Shenghong WangJing Wang
Oct 30, 2020·International Journal of Molecular Sciences·Elena V MitroshinaMaria V Vedunova
Jan 7, 2021·Environmental Science and Pollution Research International·Neicí Cáceres SilvaRobson Luiz Puntel
Apr 22, 2018·Brain Research Bulletin·Cailing LuSongchao Guo

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