Protective Effect of Exacum lawii on Cisplatin-induced Oxidative Renal Damage in Rats

Pharmacognosy Magazine
Sonam SharmaSiva Hemalatha

Abstract

Exacum lawii (Gentianaceae), a bitter herb conventionally used in kidney diseases and eye problems, endemic to the Western coast and Southern part of India. Folklore reports encourage the author to explore the nephroprotective effect of the standardized ethanolic extract of E. lawii against cisplatin-induced renal toxicity in the rat to scientifically validate its traditional use. Ethanolic extract of the whole plant of E. lawii was standardized with swertiamarin (secoiridoid glycoside) using high-performance liquid chromatography and tested for subacute toxicity according to the OECD guidelines. Nephroprotective potential at different doses of extract was evaluated against cisplatin (6 mg/kg, intraperitoneal) in experimental rats. The changes in serum renal toxicity markers, renal tissue oxidative stress biomarkers, and proinflammatory cytokines level were measured. To estimate the change in oxidative status of renal tissues, DNA and single viable cells were isolated from treated rat kidney, DNA fragmentation assay and flow cytometric analysis of reactive oxygen species (ROS) were performed. Histopathology of renal tissues was also examined. Swertiamerin was found to be 119.59 mg/g of extract. Administration of E. lawii extrac...Continue Reading

Methods Mentioned

BETA
electrophoresis
ELISA
flow cytometry
FACS
MDA
enzyme-linked immunosorbent assay

Software Mentioned

Cell Quest Pro
GraphPad
waters Breeze
Graph Pad Prism
Cell
Quest Pro

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