Protective Effect of Gentianine, a compound from Du Huo Ji Sheng Tang, against Freund's Complete Adjuvant-Induced Arthritis in Rats

Inflammation
Chen Wenjin, Wang Jianwei

Abstract

The anti-inflammatory property of gentianine (GE) aroused our interest. To investigate the effect of GE on Freund's complete adjuvant (FCA)-induced arthritis in rats was the aim of this study. Adjuvant-induced arthritis (AA) was induced by the administration of 0.1 ml FCA in the surface of hind paw. Diclofenac sodium (DS) and Fasudil were administered as positive controls. Downregulation of cytokines including interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) revealed anti-inflammatory effect of GE, which was consisted with pathological conditions of rat paws. In addition, western blot analysis revealed that GE could strikingly suppressed the expressions of Rho/NF-κB signaling pathways as well as TGF-β/smad-3 pathways. In conclusion, data indicated that GE might contribute to treat arthritis as a potential therapeutic candidate.

References

Jul 8, 1999·Journal of Medicinal Chemistry·R C Newton, C P Decicco
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
May 31, 2003·Nature Reviews. Drug Discovery·Josef S Smolen, Günter Steiner
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuo NikiNorihiro Tada
Apr 3, 2004·Arthritis Research & Therapy·Christoph SchrammManfred Blessing
Nov 5, 2008·The Journal of Clinical Investigation·Marc Feldmann
Oct 7, 2009·International Immunopharmacology·Jalal A JazayeriAnn B Vernallis
Jan 24, 2015·International Immunopharmacology·Tong ChenHui Ji
Jun 11, 2016·International Immunopharmacology·Zhiheng HuangWeiwei Tao
Aug 21, 2016·Annals of the Rheumatic Diseases·Ting Yu Wang, Di Chen

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Citations

Mar 29, 2020·International Journal of Radiation Biology·Mona A El-GhazalySanaa A Kenawy
Mar 7, 2020·Journal of Basic and Clinical Physiology and Pharmacology·Doha A MohamedKarem Fouda
Apr 8, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Sicong LiLei Bi

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Methods Mentioned

BETA
ELISA
electrophoresis

Software Mentioned

GraphPad Prism

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