PMID: 25744834Mar 7, 2015Paper

Protective effect of hydrogen sulfide on rats with myocardial ischemia/reperfusion injury and its mechanism

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Yansheng ZhangXiaohua Yu

Abstract

To explore the protective effect of hydrogen sulfide (H2S) on rats with myocardial ischemia/reperfusion injury (MIRI) and its related mechanism. Thirty male SD rats were randomly divided into 3 groups: control group, model group and H2S-treated group (n=10 in each). The MIRI model was established by ligating left anterior descending coronary artery for 40 minutes followed by reperfusion for 2 hours. Rats in H₂S-treated group were injected with 14 μmol/kg sodium hydrosulfide (NaHS) intraperitoneally before ischemia and reperfusion, respectively. After 24 hours of reperfusion, blood was collected from abdominal aorta and serum was separated. Then, the heart was removed. The myocardial histopathological changes were observed using HE staining. Serum levels of creatine kinase MB isozyme (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin I (cTnI), as well as the activities of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px) and catalase (CAT) in the homogenate of myocardium were examined using the kits. Myocardial interleukin (IL)-1β, IL-6, IL-18 and tumor necrosis factor-α (TNF-α) contents were detected by ELISA. The levels of intracytoplasm Keap 1 and intranuclear nuclear factor-erythroid 2...Continue Reading

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