Protective effect of hydroxychloroquine on rheumatoid arthritis-associated atherosclerosis

Animal Models and Experimental Medicine
Na ShiHaitao Niu

Abstract

Patients with rheumatoid arthritis (RA) have an increased risk for cardiovascular disease. We examined the effect of gut microbiota in a mouse model of RA that develops atherosclerosis. We created three groups of K/BxN female mice that were positive for the anti-glucose-6-phosphate isomerase (GPI) antibody: control diet (CD), high fat diet (HFD), and HFD with hydroxychloroquine (HFD + HCQ). Serological tests were used to detect the serum levels of total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), anti-GPI antibody titers, and serum cytokines. Atherosclerotic plaque was determined by histological analysis, and gut microbiota were determined by 16sV4 sequencing. Relative to mice given the CD, those receiving the HFD had increased serum levels of LDL-C, TCHO, and TG, decreased serum levels of HDL-C, increased atherosclerotic lesions in the aortic root, and altered gut microbiota. Addition of HCQ to HFD decreased the serum levels of LDL-C, TCHO, and TG, increased serum levels of HDL-C, and decreased the atherosclerotic lesions in the aortic root. Mice receiving HFD + HCQ also had the greatest bacterial diversity among the three experimental groups...Continue Reading

References

Oct 1, 1993·Seminars in Arthritis and Rheumatism·R I Fox
Jun 1, 1997·Annals of the Rheumatic Diseases·R MunroH A Capell
Apr 17, 2002·The Journal of Experimental Medicine·Mariana MaccioniDiane Mathis
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·Muriel DerrienWillem M de Vos
Aug 9, 2005·Seminars in Arthritis and Rheumatism·Miguel A Gonzalez-GayJavier Martin
Aug 20, 2008·Nature Clinical Practice. Rheumatology·Peter C Taylor
Sep 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Malin E V JohanssonGunnar C Hansson
Dec 24, 2008·Annals of the Rheumatic Diseases·G Ruiz-IrastorzaM A Khamashta
Jan 14, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Richard KellermayerC Wayne Smith
Jun 28, 2012·Annals of the Rheumatic Diseases·Shawn RoseHarris Perlman
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Amandine EverardPatrice D Cani
Oct 15, 2013·International Immunopharmacology·Cristen B ChafinChristopher M Reilly
Mar 25, 2016·International Journal of Molecular Sciences·Xiaohao WuGe Zhang
Jan 11, 2017·Acta Scientiarum Polonorum. Technologia Alimentaria·Iwona Węgielska, Joanna Suliburska
Mar 10, 2017·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Jose Felix RestrepoAgustin Escalante
Apr 30, 2017·Seminars in Immunopathology·Kim Lauper, Cem Gabay
Mar 11, 2018·Atherosclerosis·Lora J KasselmanAllison B Reiss

❮ Previous
Next ❯

Citations

Dec 15, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Stefano CelottoJacopo Demurtas
Oct 30, 2020·Biomolecules·Oscar Zaragoza-GarcíaIris Paola Guzmán-Guzmán

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
flow cytometry
PCR
ELISA
Illumina sequencing

Software Mentioned

Image
UPARSE
Flow Jo
GraphPad Prism
Pro Plus

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.