PMID: 20629310Jul 16, 2010Paper

Protective effect of morphine hypoxic preconditioning on liver against acute hypoxic-reoxygenation injury in rabbits

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Xiaoqin JiangHuafeng Li

Abstract

To investigate the protective effect of morphine hypoxic preconditioning on hepatic hypoxic/reoxygenation injury in rabbits and the mechanisms involved. Hypoxic/reoxygenation injury was induced with inhalation of 8% O2 for 3 hours followed by air for 3 hours. Thirty male white New Zealand rabbits were randomly divided into 3 groups: normal control group (N group), hypoxic/reoxgenation group (H/R group) and morphine hypoxic preconditioning group (MO + H/R group). Animals in the H/R and MO + H/R groups received 5 mL of saline or 3 mg/kg of morphine respectively before the induction of hypoxic injury. Hepatic apoptosis was determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) methods. The activity of superoxide dismulase (SOD) in liver tissues was measured at the end of reoxgygenation. A large number of TUNEL positive cells [(9.50 +/- 1.00)%] were observed in hepatic tissues of rabbits in the H/R group. The administration of morphine exerted a significant anti-apoptotic effect, as evidenced by reduced TUNEL-positive staining [(2.20 +/- 0.40)%, P < 0.05 vs. H/R group]. Compared with the H/R group, treatment with morphine increased SOD activity significantly [(85.57 +/- 19.37) vs....Continue Reading

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