Protective effect of N-acetyl-serotonin on the nonenzymatic lipid peroxidation in rat testicular microsomes and mitochondria

Journal of Pineal Research
M B Gavazza, Angel Catalá

Abstract

N-acetyl-serotonin, the immediate precursor of melatonin in the tryptophan metabolic pathway in the pineal gland, has been reported to be an antioxidant. The aim of this study was to test the in vitro protective effect of N-acetyl-serotonin on the ascorbate-Fe(++) induced lipid peroxidation of polyunsaturated fatty acids (PUFAs) located in testis microsomes and mitochondria. We assayed increasing concentrations (0-10 mM) of N-acetyl-serotonin in testis microsomes and (0-1 mM) of N-acetyl-serotonin in testis mitochondria. Control experiments were performed by incubating microsomal and mitochondrial membranes with N-acetyl-serotonin in the absence of lipid peroxidation-inducing drugs. Special attention was paid to the changes produced on the highly PUFAs C20:4 n6 and C22:5 n6. The light emission (chemiluminescence) used as a marker of lipid peroxidation was similar in both organelles when the control and peroxidized groups were compared. N-acetyl-serotonin reduced lipid peroxidation in testicular microsomes or mitochondria for both C20:4 n6 and C22:5 n6. Both long chain PUFAs were protected when N-acetyl-serotonin was incorporated either into microsomes or mitochondria. The N-acetyl-serotonin concentration required to inhibit by ...Continue Reading

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Citations

Dec 4, 2010·Antioxidants & Redox Signaling·Jeffrey K Yao, Matcheri S Keshavan
Sep 3, 2013·International Journal of Molecular Sciences·Shuna YuXin Wang
Oct 2, 2009·Oxidative Medicine and Cellular Longevity·R John Aitken, Shaun D Roman
Sep 18, 2007·Cell Biochemistry and Function·Adam Gesing, Malgorzata Karbownik-Lewinska
Jan 31, 2006·Journal of Pineal Research·Patricia J LardoneAna Coto-Montes
Apr 20, 2006·The International Journal of Biochemistry & Cell Biology·Angel Catalá
Jul 12, 2014·Oxidative Medicine and Cellular Longevity·Jiying JiangXin Wang
Nov 15, 2019·Journal of Receptor and Signal Transduction Research·Hasti SudhamaniWudayagiri Rajendra

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