Protective effect of puerarin against burn-induced heart injury in rats

Experimental and Therapeutic Medicine
Junling LiuHui Wang

Abstract

The present study evaluated the potential protective effects of puerarin and its associated mechanism on burn-induced myocardial damage. A total of 40 healthy adult Wistar rats were randomly divided into four groups: i) Sham; ii) burn; iii) burn + puerarin; and iv) puerarin. Serum levels of interleukin (IL)-1β, tumor necrosis factor-α (TNF-α) and IL-6 were measured using ELISA. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels were determined in myocardial homogenates using a commercial assay kit. TUNEL staining and western blot analysis of cleaved and pro-caspase-3 were also performed to assess apoptosis. Activation of p38-MAPK, ERK, JNK and AKT were measured using western blot analysis. Left ventricular systolic pressure, maximum rates of increase/decrease in left ventricular pressure, creatine kinase MB activity and cardiac troponin T levels were found to be altered in the burn group 12 h after burn, which were reversed by puerarin treatment. Injection of puerarin following burn injury also reduced heart water content. Serum levels of IL-1β, TNF-α and IL-6 were significantly higher in the burn group compared with those in the sham group. Puerarin treatment reduced serum levels of IL-1β, TNF-α and IL-6, in addit...Continue Reading

References

Apr 9, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·E T RietschelA J Ulmer
Jul 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·A C CarrB Frei
Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A T SaurinM S Marber
Dec 15, 2000·Current Opinion in Structural Biology·M G Grütter
Apr 24, 2001·Pharmacology & Therapeutics·S K Wattanapitayakul, J A Bauer
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·G S De Zutter, R J Davis
Nov 22, 2001·JAMA : the Journal of the American Medical Association·R ZhangS L Hazen
Jun 8, 2002·Burns : Journal of the International Society for Burn Injuries·F L Korompai, S Y Yuan
Jun 29, 2002·Science·Jason P EiserichBruce A Freeman
Jul 1, 1961·Annals of Surgery·H A FOZZARD
Oct 19, 2004·Shock·Jureta W HortonJoseph Murphy
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Daniel R MeldrumKirstan K Meldrum
Jul 1, 2005·Shock·Jureta W HortonCherry Ballard-Croft
Mar 24, 2007·Osteoarthritis and Cartilage·R Wong, B Rabie
May 1, 2007·Burns : Journal of the International Society for Burn Injuries·Yuesheng HuangXiaorong Zhang
Jul 27, 2007·The Journal of Pharmacology and Experimental Therapeutics·Xiao-Hui SunHui-Bi Xu
Sep 18, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L WuL Li
Aug 13, 2008·The Journal of Trauma·Jia-Ping ZhangWen-Chang Wang
Apr 14, 2009·Free Radical Biology & Medicine·Britta U GoldmannStephan Baldus
Apr 14, 2010·The American Journal of Chinese Medicine·Min ZhaoNa-Na Wang
Oct 7, 2010·Journal of Asian Natural Products Research·Zhi-Qiang FengPiao-Yang Sun
Apr 2, 2011·Chemico-biological Interactions·Shao-Zhen HouXiao-Ping Lai
Aug 16, 2011·Journal of Burn Care & Research : Official Publication of the American Burn Association·Gen-fa LvXiong-xiang Zhu
May 15, 2013·Microvascular Research·Jochen Frederick HernekampThomas Kremer
Aug 3, 2013·Journal of cardiology·Yuan YuanQi-Zhu Tang
Dec 18, 2013·Phytotherapy Research : PTR·Yan-Xi ZhouCheng Peng
Jan 28, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Xiao YaoQun S Zang
Dec 23, 2014·Immunology Letters·Jing WangShumin Wang
Oct 31, 2015·Burns : Journal of the International Society for Burn Injuries·Sheng LiuYong-Jie Wang
Jan 6, 2016·International Journal of Molecular Sciences·Ashley N GuilloryCeleste C Finnerty
Apr 19, 2017·Journal of Clinical Periodontology·Luiz Felipe C FrançaDaniel F P Vasconcelos
Jul 27, 2017·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Verena Sampaio Barbosa MatosMarcos Lázaro da Silva Guerreiro
Jan 30, 2018·Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns·Y S Huang
Jun 30, 2018·Anesthesiology·Sabri SoussiMatthieu Legrand
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yu ZhangFayong Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
light microscopy
protein assay
electrophoresis
MDA

Software Mentioned

ImageJ
GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis