Protective effect of remote limb ischemic perconditioning on the liver grafts of rats with a novel model

PloS One
Junjun JiaShusen Zheng

Abstract

Remote ischemic conditioning (RIC) is a known manual conditioning to decrease ischemic reperfusion injury (IRI) but not increase ischemic time. Here we tried to establish a rat RIC model of liver transplantation (LT), optimize the applicable protocols and investigate the protective mechanism. The RIC model was developed by a standard tourniquet. Sprague-Dawley rats were assigned randomly to the sham operated control (N), standard rat liver transplantation (OLT) and RIC groups. According to the different protocols, RIC group was divided into 3 subgroups (10 min×3, n = 6; 5 min×3, n = 6; 1 min×3, n = 6) respectively. Serum transaminases (ALT, AST), creatine kinase (CK), histopathologic changes, malondialdehyde (MDA), myeloperoxidase (MPO) and expressions of p-Akt were evaluated. Compared with the OLT group, the grafts subjected to RIC 5min*3 algorithm showed significant reduction of morphological damage and improved the graft function. Also, production of reactive oxygen species (MDA) and neutrophil accumulation (MPO) were markedly depressed and p-Akt was upregulated. In conclusion, we successfully established a novel model of RIC in rat LT, the optimal RIC 5min*3 algorithm seemed to be more efficient to alleviate IRI of the live...Continue Reading

References

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Citations

Jun 14, 2017·The Journal of Trauma and Acute Care Surgery·Alexander J SandweissBellal Joseph
Feb 15, 2018·The Journal of Trauma and Acute Care Surgery·Viraj PanditBellal Joseph
Sep 28, 2016·World Journal of Gastroenterology : WJG·Niteen TapuriaBrian R Davidson
Jun 15, 2021·Molecular and Cellular Biochemistry·Martín DonatoRicardo J Gelpi
Jun 17, 2021·World Journal of Hepatology·Rafał Stankiewicz, Michał Grąt

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Methods Mentioned

BETA
MDA

Software Mentioned

R
IPER

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