Protective effect of retinal ischemia by blockers of voltage-dependent calcium channels and intracellular calcium stores

Cellular and Molecular Neurobiology
Pindaro Dias MassoteMarcus Vinicius Gomez

Abstract

In the present study, the neuroprotective effect of blockers of voltage-dependent calcium channels (VDCC) and intracellular calcium stores on retinal ischemic damage induced by oxygen deprivation-low glucose insult (ODLG) was investigated. Retinal damage induced by ODLG was dependent on the calcium concentration in the perfusion medium. When incubated in medium containing 2.4 mM CaCl(2), cell death in ischemic retinal slices treated with blockers of VDCC, omega-conotoxin GVIA (1.0 microM), omega-conotoxin MVIIC (100 nM) and nifedipine (1.0 microM), was reduced to 62 +/- 2.3, 46 +/- 4.3 and 47 +/- 3.9%, respectively. In the presence of blockers of intracellular calcium stores, dantrolene (100 microM) and 2-APB (100 microM), the cell death was reduced to 46 +/- 3.2 and 55 +/- 2.9%, respectively. Tetrodotoxin (1.0 microM), reducing the extent of the membrane depolarization reduces the magnitude of calcium influx trough VDCC causing a reduction of the cell death to 55 +/- 4.3. Lactate dehydrogenase content of untreated ischemic retinal slices was reduced by 37% and treatment of ischemic slices with BAPTA-AM (100 microM) or 2-APB (100 microM) abolished the leakage of LDH. Dantrolene (100 microM) and nifedipine (1.0 microM) partially...Continue Reading

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Citations

Jun 22, 2013·Expert Opinion on Medical Diagnostics·Bruce A BerkowitzRobin Roberts
Dec 16, 2011·Journal of Neurochemistry·Davide CerviaGiovanni Casini
Aug 16, 2011·Toxicology Letters·Marong FangYu Geng
Apr 18, 2018·Clinical Hemorheology and Microcirculation·Hai-Peng LinPing-Fan Guo
Apr 8, 2010·Expert Review of Molecular Diagnostics·Harald Kolmar
Oct 22, 2015·Reviews in the Neurosciences·Ephrem EngidaworkGert Lubec

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