Protective effect of S-adenosyl-L-methionine on bromobenzene- and D-galactosamine-induced toxicity to isolated rat hepatocytes

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
J WuA Danielsson

Abstract

The protective effect of S-adenosyl-L-methionine (SAMe) on bromobenzene (BB)- or D-galactosamine (GalN)-induced damage to isolated rat hepatocytes and its effect on cellular glutathione (GSH) levels were investigated. SAMe at concentrations of 0.5 to 3.0 mmol/L significantly reduced lactate dehydrogenase leakage from cells exposed to 1.6 mmol/L BB (P < .05 to .001) during 2 hours' incubation. GalN at 25 to 50 mmol/L induced a marked increase of LDH leakage from the cells during the later stage of 24 hours' incubation and SAMe at 1.0 mmol/L clearly attenuated the LDH leakage in GalN (25 mmol/L)-exposed cells. The GSH content in the cells exposed to 2.4 mmol/L BB for 150 minutes was markedly decreased, and further decreased during 24 hours' incubation. SAMe (1.5 mmol/L) both reduced LDH leakage and corrected GSH depletion in cells exposed to 2.4 mmol/L BB. The GSH content in 25 and 50 mmol/L GalN-exposed cells was strikingly diminished to 51.2% and 32.8% of the controls, respectively, during 24 hours' of exposure. SAMe at 1.5 mmol/L significantly reduced the loss in GSH content in 25 mmol/L GalN-exposed cells. The findings show that SAMe has beneficial effects on both BB- and GalN-induced toxicity to rat hepatocytes. The main mec...Continue Reading

Citations

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