Abstract
This study was designed to investigate possible protective effects of sevoflurane on myocardial ischemia-reperfusion injury (MIRI) and its impact on expression of HIF-1α and caspase-3 in rats, so as to provide new insights for the treatment of MIRI. Forty SD rats were randomly divided into four groups (n=10) including Sham operation (Sham), ischemia-reperfusion (IR), sevoflurane preconditioning group (Sevo-Pre) and sevoflurane post-conditioning (Sevo-Post) groups. Perfusion was performed using ex vivo heart perfusion. The baseline values of cardiac function were recorded in each group at the end of balanced perfusion and after 60 min of reperfusion. Myocardial infarct size (MIS) was calculated at the end of perfusion using TTC staining. Levels of HIF-1α and caspase-3 protein and HIF-1α (western blotting) and Bcl-2 mRNA (RT-qPCR) were detected at the end of reperfusion. Our results showed no significant differences in cardiac function between the groups at the end of the balanced perfusion. After reperfusion for 60 min, however, the cardiac functions of the Sevo-Pre and Sevo-Post groups were significantly better than those in the IR group, and the MIS at the end of reperfusion was significantly decreased. Western blotting and RT...Continue Reading
References
Jul 1, 1992·Hypertension·J I Goldhaber, J N Weiss
Feb 28, 2002·Biochemical and Biophysical Research Communications·Louis X LiuCanwen Jiang
Sep 10, 2002·Anesthesiology·Franz KehlJudy R Kersten
Feb 14, 2004·Cardiovascular Research·Frank EeftingPieter A Doevendans
Aug 18, 2004·Biochemical Pharmacology·Nathalie M MazureJacques Pouysségur
Jul 23, 2005·Anesthesia and Analgesia·Mitsuharu KodakaPeter S Sebel
Jul 30, 2005·Anesthesiology·Guochang HuGeorge J Crystal
Dec 6, 2005·Journal of the American College of Cardiology·Masakuni KidoPatricia A Thistlethwaite
Jun 20, 2006·Cardiovascular Research·Joachim FandreyMax Gassmann
Jul 19, 2006·The Journal of Biological Chemistry·Xiang-Hong PengLily Yang
Nov 17, 2007·Cardiovascular Research·Zheqing CaiGregg L Semenza
Mar 27, 2012·Molecular Biology Reports·Jun-Song GongLi-Huan Li
Jun 5, 2013·Journal of Zhejiang University. Science. B·Yuan-yuan YaoMin Yan
Nov 13, 2013·Biochemical and Biophysical Research Communications·Xueming CaoYunpeng Li
Jan 28, 2014·PloS One·Zhihua HanChangqian Wang
Feb 11, 2014·Asian Pacific Journal of Tropical Medicine·Qian HuHeng-Fang Liu
Mar 19, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Anzhou XiaWeidong Zhou
Apr 12, 2014·PloS One·Xiang-Yan MengRui-Cheng Xu
Citations
May 28, 2019·Journal of Cellular Biochemistry·Xing GeZhenhua Shi
Jan 29, 2021·BioMed Research International·Alessandro BertaniAntonio Arcadipane
May 14, 2021·Aging·Tao LiBaoguo Ye
Oct 23, 2021·Experimental and Therapeutic Medicine·Hefei WangShijiang Sun