Protective Effect of Stachydrine Against Cerebral Ischemia-Reperfusion Injury by Reducing Inflammation and Apoptosis Through P65 and JAK2/STAT3 Signaling Pathway

Frontiers in Pharmacology
Li LiJunqin Mao

Abstract

Stachydrine, a constituent of Leonurus japonicus Houtt which also called Japanese motherwort has been shown to improve vascular microcirculation and ameliorate endothelial dysfunction. This study investigated the neuroprotective effect of stachydrine. Male Sprague-Dawley (SD) rats were randomly divided into sham, control, and stachydrine groups. The neurological deficit score was evaluated and the infarct size of the brain was measured using 2,3,5-triphenyltetra-zolium (TTC) chloride staining assay, and the pathological changes in the brain tissues were examined by HE staining. Nissl and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) staining were performed to assess the numbers of Nissl bodies and the levels of apoptosis in the neurons. The activities of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) were also measured. The release of inflammatory factors IL-1β and TNF-α were detected by Enzyme-linked immunosorbent assay (ELISA). Compared with the control group, the stachydrine group showed a significant prevention of neurological deficit, as indicated by the reduced infarct volume in the brain. Moreover, the stachydrine treatment reduced the activities of SOD, the...Continue Reading

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Citations

Jul 1, 2020·Neural Regeneration Research·Yan-Qing HuangA-Li Yang
Jan 16, 2021·Advanced Healthcare Materials·Anthony BrouillardAshish A Kulkarni
Apr 15, 2021·Journal of Molecular Neuroscience : MN·Chun LuoYinghai Wei

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Methods Mentioned

BETA
ELISA
Assay
Enzyme-Linked Immunosorbent Assay
electrophoresis
confocal microscopy
MDA
flow cytometry
PCR

Software Mentioned

Pro Plus
SPSS19
Image

Related Concepts

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