Protective effect of the Baicalin against DNA damage induced by ultraviolet B irradiation to mouse epidermis

Photodermatology, Photoimmunology & Photomedicine
Zhou Bing-RongLuo Dan

Abstract

To investigate whether topical application of Baicalin affords protecting Balb/C mice epidermis from ultraviolet (UV)B-induced DNA damage and its underlying mechanisms. A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining, Southwestern dot-blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; Western blotting was used for p53 detection; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of Bcl-2 and Bax; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells. Topical application of Baicalin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 48 h after 180 mJ/cm(2) of UVB irradiation as compared with untreated mice. UVB-induced apoptosis was less pronounced in Baicalin-treated mice epidermis, which was accompanied by less p53 accumulation and higher Bcl-2/Bax ratio compared with that of untreated mice. Taken together, these results suggest that topical Baicalin application mitigates DNA photo-damage. Baicalin is therefore a promising protective substance against UVB radiation.

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Citations

Apr 11, 2008·Archives of Dermatological Research·Bing-Rong ZhouJie Gao
Dec 15, 2010·Archives of Biochemistry and Biophysics·Farrukh Afaq
Oct 1, 2013·Journal of Neurotrauma·Ying WangJoseph B Long
Nov 20, 2012·Journal of Dermatological Science·Ze GuoDan Luo
Jan 12, 2010·International Journal of Pharmaceutics·Yi-Hung TsaiPao-Chu Wu
Sep 15, 2009·Photodermatology, Photoimmunology & Photomedicine·Bing-Rong ZhouDan Luo
Mar 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ki Cheon KimJin Won Hyun
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Dec 26, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yanping ZhuHairong Lin
Jan 29, 2013·International Journal of Oncology·Chingju LinJai-Sing Yang
Jan 11, 2020·ACS Applied Materials & Interfaces·Yuchun QiaoXueji Zhang
Jul 3, 2021·International Journal of Molecular Sciences·Anna Domaszewska-SzostekAlina Kuryłowicz

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