Protective effect of the DNA vaccine encoding the major house dust mite allergens on allergic inflammation in the murine model of house dust mite allergy

Clinical and Molecular Allergy : CMA
Nacksung KimTai June Yoo

Abstract

Vaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells. To evaluate the effects of vaccination with mixed naked DNA plasmids encoding Der p 1, Der p 2, Der p 3, Der f 1, Der f 2, and Der f 3, the major house dust mite allergens on the allergic inflammation to the whole house dust mites (HDM) crude extract. Three hundred micrograms of these gene mixtures were injected into muscle of BALB/c mice. Control mice were injected with the pcDNA 3.1 blank vector. After 3 weeks, the mice were actively sensitized and inhaled with the whole house dust mite extract intranasally. The vaccinated mice showed a significantly decreased synthesis of total and HDM-specific IgE compared with controls. Analysis of the cytokine profile of lymphocytes after challenge with HDM crude extract revealed that mRNA expression of interferon-gamma was higher in the vaccinated mice than in the controls. Reduced infiltration of inflammatory cells and the prominent infiltration of CD8+ T cells were observed in histology of lung tissue from the vaccinated mice. Vaccination with DNA encoding the major house dust mite allergens provides a promising approach for treating allergic response...Continue Reading

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Citations

Oct 26, 2012·Human Vaccines & Immunotherapeutics·Philippe Moingeon
Nov 2, 2012·Allergy, Asthma & Immunology Research·Kyoung Yong JeongChein-Soo Hong
Oct 23, 2008·Current Allergy and Asthma Reports·Noel Rodríguez-PérezJay M Portnoy
Nov 30, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y Waeckerle-MenP Johansen
Oct 3, 2006·Current Opinion in Immunology·Reto Crameri, Claudio Rhyner

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Methods Mentioned

BETA
PCR
ELISA
FCS
light microscopy
bronchoalveolar lavage

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