Abstract
Previous studies have shown that free polyunsaturated fatty acids (PUFA) reduce the excitability of cardiac myocytes and exert antiarrhythmic effects. Therefore, we hypothesized that retinoic acid (RA, vitamin A acid), which has structural characteristics similar to those of PUFA, may have similar antiarrhythmic effects. To test this hypothesis, we used an isolated, spontaneously beating, neonatal rat cardiac myocyte preparation to examine the effects of RA, added to the perfusion solution, on the cell contraction and arrhythmias induced by isoproterenol (ISO) or lysophosphatidylcholine (LPC). All-trans-RA (10-20 microM) induced a marked and reversible reduction in the contraction rate of the cell in 2-5 min without changing the amplitude of the contractions. Superfusion of the myocytes with either ISO (3 microM) or LPC (5 microM) induced sustained tachyarrhythmias characterized by spasmodic contractures and fibrillation. Addition of 15-20 microM all-trans-RA to the perfusion solution effectively prevented as well as terminated the arrhythmias induced by ISO and LPC. Furthermore, in a whole-animal model of arrhythmia in which the left anterior descending coronary artery (LAD) of the anesthetized rat was occluded for 15 min foll...Continue Reading
Citations
Sep 19, 2009·Cardiovascular Toxicology·Rajat SethiNaranjan S Dhalla
Aug 1, 1997·Prostaglandins·Y LiA Leaf
Nov 13, 2004·The Journal of Nutritional Biochemistry·K NageswariV P Menon
Nov 13, 2004·The Journal of Nutritional Biochemistry·W R LeifertE J McMurchie
Nov 1, 1997·Trends in Cardiovascular Medicine·S M Smith, E D Dickman
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·J X Kang, A Leaf
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y F XiaoA Leaf
Feb 24, 2006·Acta Pharmacologica Sinica·Dan-yan Zhu, Yi-jia Lou
Mar 3, 2015·Journal of the Chinese Medical Association : JCMA·Chen-Hsiang Shih, Hung-Bo Wu
Mar 1, 2006·International Journal of Cardiology·Nipon ChattipakornSiriporn C Chattipakorn
Oct 1, 1996·Circulation·J X Kang, A Leaf
Jun 17, 2018·The Journal of Pharmacology and Experimental Therapeutics·Alice MarinoRoberto Levi
Apr 29, 2014·Molecular and Cellular Biochemistry·Yangzhen ShaoElmir Omerovic
Jul 10, 2018·Case Reports in Hematology·Pin-Zi ChenYu-Wen Wang
Jan 5, 2000·The American Journal of Clinical Nutrition·J X Kang, A Leaf