Protective effects of aloin on oxygen and glucose deprivation-induced injury in PC12 cells

Brain Research Bulletin
Ren-Yuan ChangJian-Qiang Yu

Abstract

The present study aims to determine whether aloin could protect cells from ischemic and reperfusion injury in vitro and to elucidate the related mechanisms. Oxygen and glucose deprivation model in PC12 cells was used in the present study. 2-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) assay and Hoechst 33342 nuclear staining were used to evaluate the protective effects of aloin, at concentrations of 10, 20, or 40 μg/mL in PC12 cells. PCR was applied to detect fluorescence caspase-3, Bax and Bcl-2 mRNA expression in PC12 cells. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) activity were evaluated by biochemical method. The concentration of intracellular-free calcium [Ca(2+)]i, mitochondrial membrane potential (MMP) were determined to estimate the degree of neuronal damage. It was shown that aloin (10, 20, and 40 μg/mL) significantly attenuated PC12 cells damage with characteristics of an increased injured cells absorbance of MTT and releases of LDH, decreasing cell apoptosis, and antagonizing decreases in SOD activity and increase in MDA level induced by OGD-reoxygenation. Meanwhile pretreatment with aloin significantly reduced injury-induced intracellular ROS,...Continue Reading

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Citations

Nov 22, 2017·Cellular and Molecular Neurobiology·Yazhou XuTao Pang
Jul 16, 2020·The Journal of Pharmacy and Pharmacology·Da-Bao ChenBiao Cai
Nov 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunsoo Jung, Junghyun Kim
May 22, 2020·Progress in Retinal and Eye Research·Stephan Ong ToneUla V Jurkunas
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jun-Jie ShiLi-Fang Yu
Jul 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Cun LiuChanggang Sun
Jun 7, 2021·Journal of Applied Toxicology : JAT·Yan-Ping LiuZhao-Hui Zhang
Jul 18, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Bayarmaa KhadankhuuYunman Li
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yue LiuJianqiang Yu

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