Protective effects of baicalein against cell damage by reactive oxygen species

Chemical & Pharmaceutical Bulletin
D GaoH Sakurai

Abstract

Baicalein (5,6,7-trihydroxy-2-phenyl-4H-1-benzopyran-4-one), a naturally occurring flavonoid, was found to prevent human dermal fibroblast cell damage induced by reactive oxygen species such as hydrogen peroxide (H2O2), tert-butyl hydroperoxide (BuOOH) and superoxide anions (.O2-) in a concentration-dependent manner, and was more effective than the iron chelator, deferoxamine, hydroxyl radical (.OH) scavengers such as dimethyl sulfoxide (DMSO) and ethanol (EtOH), the lipid peroxidation chain blocker, alpha-tocopherol (Vit. E) and the xanthine oxidase inhibitor, allopurinol. To probe the mechanism of cell defense, the reaction of baicalein with oxygen free radicals was investigated using electron spin resonance (ESR) spectrometry. Baicalein decreased the signal intensities due to the 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) spin adducts of .OH, .O2- and tert-butyl peroxyl (BuOO.) radicals in a concentration-dependent manner. The IC50 values, which are the 50% inhibition concentrations of baicalein for the free radicals, were 10, 45 and 310 microM, respectively. These results suggested that baicalein possesses free radical scavenging ability which prevents the fibroblast damage induced by these free radical species.

Citations

Oct 15, 2014·Journal of Molecular Modeling·Houria DjeradiAbdelkrim Cheriti
Dec 22, 2005·AIDS Research and Therapy·Han AungChun-Su Yuan
Oct 20, 1999·Journal of Molecular and Cellular Cardiology·Z H ShaoC S Yuan
Apr 6, 2005·World Journal of Gastroenterology : WJG·Xi-Ping ZhangYi-Feng Zhou
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcello IritiFranco Faoro
Jan 17, 2012·Cellular and Molecular Neurobiology·Maryam MoslehiRazieh Yazdanparast
Feb 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Zuo-Hui ShaoChun-Su Yuan

Related Concepts

Baicalein
5,5-dimethyl-1-pyrroline-1-oxide
Cyclic N-Oxides
Dose-Response Relationship, Drug
Electron Spin Resonance Spectroscopy
Fibroblasts
Bioflavonoids
Perhydrol
Spin Labels
Superoxides

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