Protective effects of catalase overexpression on UVB-induced apoptosis in normal human keratinocytes

The Journal of Biological Chemistry
Hamid Reza RezvaniHubert de Verneuil

Abstract

UV-induced apoptosis in keratinocytes is a highly complex process in which various molecular pathways are involved. These include the extrinsic pathway via triggering of death receptors and the intrinsic pathway via DNA damage and reactive oxygen species (ROS) formation. In this study we investigated the effect of catalase and CuZn-superoxide dismutase (SOD) overexpression on apoptosis induced by UVB exposure at room temperature or 4 degrees C on normal human keratinocytes. Irradiation at low temperature reduced UV-induced apoptosis by 40% in normal keratinocytes independently of any change in p53 and with a decrease in caspase-8 activation. Catalase overexpression decreased apoptosis by 40% with a reduction of caspase-9 activation accompanied by a decrease in p53. Keeping cells at low temperature and catalase overexpression had additive effects. CuZn-SOD overexpression had no significant effect on UVB-induced apoptosis. UVB induced an increase in ROS levels at two distinct stages: immediately following irradiation and around 3 h after irradiation. Catalase overexpression inhibited only the late increase in ROS levels. We conclude that catalase overexpression has a protective role against UVB irradiation by preventing DNA damag...Continue Reading

References

Jan 17, 1995·Biochemical and Biophysical Research Communications·H MasakiH Sakurai
Jan 1, 1995·Biochemical Society Symposium·R M Tyrrell
Oct 25, 1996·Journal of Molecular Biology·W T SteegengaA G Jochemsen
Nov 5, 1997·The Journal of Biological Chemistry·A RehemtullaV M Dixit
May 2, 1998·The EMBO Journal·C ScaffidiM E Peter
Jun 25, 1998·The Journal of Cell Biology·S TanD Schubert
Oct 9, 1998·Genes & Development·A J Giaccia, M B Kastan
Oct 31, 1998·Current Opinion in Immunology·M E Peter, P H Krammer
May 8, 1999·The Journal of Investigative Dermatology·D PeusM R Pittelkow
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·D KulmsT Schwarz
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Feb 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·H StegeJ Krutmann
Aug 6, 2000·Free Radical Biology & Medicine·K HensleyR A Floyd
Jun 16, 2001·Experimental Dermatology·G MurphyT Schwarz
Aug 18, 2001·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·H SauerJ Hescheler
Jul 12, 2002·Molecular Carcinogenesis·Samar MaaloufHala Gali-Muhtasib
Oct 26, 2002·Free Radical Biology & Medicine·Carine MichielsMartine Raes
Dec 7, 2002·Journal of Photochemistry and Photobiology. B, Biology·Muriel Cario-AndréAlain Taïeb
Sep 16, 2003·Journal of Cell Science·Susan HauptYgal Haupt
Dec 4, 2003·Journal of Photochemistry and Photobiology. B, Biology·N MorleyD Gould
Aug 3, 2004·Nature Reviews. Cancer·Hitoshi Okada, Tak W Mak
Mar 8, 2005·Free Radical Biology & Medicine·Hongjun Wang, Irene E Kochevar
Sep 10, 2005·Apoptosis : an International Journal on Programmed Cell Death·R TakasawaS Tanuma

❮ Previous
Next ❯

Citations

Mar 7, 2013·Archives of Dermatological Research·Hongfu XieJi Li
Aug 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Huan Ling LiangVani Nilakantan
Oct 15, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Konrad KleszczyńskiTobias W Fischer
Mar 17, 2011·Journal of Agricultural and Food Chemistry·Cecilia MatitoMarta Cascante
Mar 4, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·An Van LaethemPatrizia Agostinis
Dec 3, 2010·The Journal of Clinical Investigation·Hamid Reza RezvaniDavid R Bickers
May 3, 2007·Biological & Pharmaceutical Bulletin·Takahiro Ishida, Ikuyo Sakaguchi
Apr 23, 2013·Cytotechnology·Xiaoguang ChenWen Deng
May 19, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Wei-Hsun WangJeng-Jong Hwang
Oct 10, 2014·The Journal of Investigative Dermatology·Mohsen HosseiniHamid R Rezvani
Dec 2, 2009·Mechanisms of Ageing and Development·Valentina StreckerMarina Jendrach
Jan 22, 2010·The Journal of Investigative Dermatology·Ho-Cheol RyuJae-Hong Kim
Dec 2, 2014·The Journal of Investigative Dermatology·Mohsen HosseiniHamid Reza Rezvani
Jul 9, 2010·The Journal of Investigative Dermatology·Ling LiuTianwen Gao
Dec 5, 2008·Experimental Dermatology·Valerie Anne TraboshDean S Rosenthal
Feb 19, 2008·Experimental Dermatology·Livius WunderlichJózsef Mandl
May 15, 2008·Photodermatology, Photoimmunology & Photomedicine·Mariela L PazJuliana Leoni
Nov 7, 2012·Photodermatology, Photoimmunology & Photomedicine·Ki Cheon KimJin Won Hyun
Nov 26, 2013·Photochemistry and Photobiology·Min YaoIrene E Kochevar
Aug 15, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Mengying JiaLi Zhang
Jul 18, 2009·International Journal of Cancer. Journal International Du Cancer·Brian C WulffTatiana M Oberyszyn
Apr 7, 2016·Journal of Cancer Prevention·Chul-Ho Jeong, Sang Hoon Joo
Oct 21, 2014·Photochemistry and Photobiology·Jean CadetJean-Luc Ravanat
Mar 17, 2015·Free Radical Biology & Medicine·Geoffroy WalbrecqBernard Knoops
Sep 3, 2010·International Journal of Radiation Biology·Alena Svobodová, Jitka Vostálová
Dec 18, 2015·Dose-response : a Publication of International Hormesis Society·Katia StankovaRayna Boteva
Jun 2, 2009·Journal of Photochemistry and Photobiology. B, Biology·XiaoJin LiuChunBo Wang
Jan 23, 2017·Free Radical Biology & Medicine·André Passaglia SchuchCamila Carrião Machado Garcia
Jan 31, 2017·The Journal of Investigative Dermatology·Houssam RaadHamid Reza Rezvani
Feb 17, 2017·The Journal of Investigative Dermatology·Jin Young BaekTong-Shin Chang
Jul 8, 2017·Proteomes·Ida Pucci-MinafraSalvatore Minafra
Mar 24, 2018·International Journal of Molecular Sciences·Pengcheng XuLin Zhang
Jan 6, 2018·Biomedicines·Ilya O VelegzhaninovOlesya M Vakhrusheva

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